Magpure Plasmid Ef Mini Kit Isolation Endotoxin Free Plasmid DNA

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: P181402/ P181403/ P181404
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, For Microscopic Purpose, Isolation Endotoxin Free Plasmid DNA Frombacterial
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Enzyme Digestion, Sequencing, PCR and Labeling
• Source: Imported Reagents
• Habit Appellation: Magpure Plasmid Ef Mini Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Enzyme Digestion, Sequencing, PCR and Labeling
• Property: Biochemical Reagent
• Main Functions: Isolation Endotoxin Free Plasmid DNA From Bacteria
• Applications: Enzyme Digestion, Sequencing, PCR and Labeling
• Purification Technology: Magnetic Beads Technology
• Process Method: Manual or Automatic
• Sample Type: Conventional Plasmid, Plasmid≤30kb
• Sample Amount: 1-5ml
• Elution Volume: ≥50μl
• Time Per Run: ≤80 Minutes
• After-Sales Service: Technical Support
• Sample: Free Sample
• Transport Package: Box
• Specification: 100 preps / 500 preps / 5000 preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

MagPure Plasmid EF Mini Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
P181402	MagPure Plasmid EF Mini Kit	100 preps	$120.00
P181403	MagPure Plasmid EF Mini Kit	500 preps	$478.00
P181404	MagPure Plasmid EF Mini Kit	5000 preps	$3,825.00

△ Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.






△ Details

Features	Specifications
Main Functions	Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid≤30KB
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤80 minutes

△ Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - it takes only 80 minutes to complete the isolation
• High yield - up to 15μg plasmid can be binded in one column
• Economic - excellent cost effectiveness performance

△  Kit Contents

Contents	P181402	P181403	P181404
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	30 mg	2 x 160 mg
Buffer P1	30 ml	150 ml	2 x 800 ml
Buffer P2	30 ml	150 ml	2 x 800 ml
Buffer LEN3	20 ml	80 ml	800 ml
Buffer LN4	90 ml	400 ml	4 x 980 ml
MagPure Particles	3.5 ml	17 ml	3 x 60 ml

△ Storage and Stability

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.





 

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