Product Description
MagPure Plasmid EF Mini Kit
| CAT NO |
PRODUCT NAME |
SIZE |
PRICE |
| P181402 |
MagPure Plasmid EF Mini Kit |
100 preps |
$120.00 |
| P181403 |
MagPure Plasmid EF Mini Kit |
500 preps |
$478.00 |
| P181404 |
MagPure Plasmid EF Mini Kit |
5000 preps |
$3,825.00 |
Introduction
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Details
| Features |
Specifications |
| Main Functions |
Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture |
| Applications |
Enzyme digestion, sequencing, PCR and labeling, etc. |
| Purification technology |
Magnetic beads technology |
| Process method |
Manual or automatic |
| Sample type |
Conventional plasmid, plasmid≤30KB |
| Sample amount |
1-5ml |
| Elution volume |
≥50μl |
| Time per run |
≤80 minutes |
Advantages
- High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
- Fast - it takes only 80 minutes to complete the isolation
- High yield - up to 15μg plasmid can be binded in one column
- Economic - excellent cost effectiveness performance
Kit Contents
| Contents |
P181402 |
P181403 |
P181404 |
| Purification Times |
100 Preps |
500 Preps |
5000 Preps |
| RNase A |
10 mg |
30 mg |
2 x 160 mg |
| Buffer P1 |
30 ml |
150 ml |
2 x 800 ml |
| Buffer P2 |
30 ml |
150 ml |
2 x 800 ml |
| Buffer LEN3 |
20 ml |
80 ml |
800 ml |
| Buffer LN4 |
90 ml |
400 ml |
4 x 980 ml |
| MagPure Particles |
3.5 ml |
17 ml |
3 x 60 ml |
Storage and Stability
RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.
Protocol
Detailed Photos
Company Profile
FAQ
Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)
Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .
Q3.Are you a manufacturer ?
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.
Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.
Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.
Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.
Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.
Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.
Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.
Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.
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