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HiPure DNA Clean Up Kit
Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D214102 | HiPure DNA Clean Up Kit | 100 preps | $60.00 |
| D214103 | HiPure DNA Clean Up Kit | 250 preps | $150.00 |
HiPure DNA Clean Up Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
| Features | Specifications |
| Main Functions | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Crude DNA, enzymatic reaction solution |
| Sample amount | <150μl |
| Recovery | 80% |
| Elution volume | ≥15μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
Kit Contents
| Contents | D214102 | D214103 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GWP | 30 ml | 60 ml |
| Buffer DW2* | 20 ml | 50 ml |
| Elution Buffer | 30 ml | 60 ml |
| HiPure DNA Mini Columns I | 100 | 250 |
| 2 ml Collection Tubes | 100 | 2 x 125 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
If any precipitates form in the buffers, warm at 37ºC to dissolve.
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