HiPure Bacterial DNA Kit
| CAT NO |
PRODUCT NAME |
SIZE |
PRICE |
| R418102 |
HiPure Bacterial RNA Kit |
50 preps |
$209.00 |
| R418103 |
HiPure Bacterial RNA Kit |
250 preps |
$801.00 |
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Specifications
| Features |
Specifications |
| Main Functions |
Isolation total RNA from bacteria culture |
| Applications |
RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Purification method |
Mini spin column |
| Purification technology |
Silica technology |
| Process method |
Manual (centrifugation or vacuum) |
| Sample type |
Bacteria culture |
| Sample amount |
Bacteria: <10^9 |
| Elution volume |
≥30μl |
| Time per run |
≤40 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column |
100µg |
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
- Fast - several samples can be extracted in 30 minutes
- High purity - the purified RNA can be directly used in various downstream applications
- High recovery - RNA can be recovered at the level of PG
- Good repeatability - silica gel column purification technology can obtain ideal results every time
- Broad spectrum - it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components - the kit contains carried lysozyme and glass beads
Kit Contents
| Contents |
R418101 |
R418102 |
R418103 |
| Purification Times |
10 Preps |
50 Preps |
250 Preps |
| gDNA Filter Mini Columns |
10 |
50 |
250 |
| HiPure RNA Mini Columns |
10 |
50 |
250 |
| 2ml Collection Tubes |
20 |
100 |
500 |
| Glass Beads (0.1-0.6mm) |
10 g |
30 g |
150 g |
| Plastic spoon |
2 |
4 |
10 |
| Lysozyme |
20 mg |
90 mg |
400 mg |
| Protease Dissolve Buffer |
1.8 ml |
1.8 ml |
10 ml |
| Buffer TE |
1.8 ml |
1.8 ml |
5 ml |
| Buffer STL |
5 ml |
20 ml |
90 ml |
| Buffer RLC |
10 ml |
30 ml |
150 ml |
| Buffer RW1 |
10 ml |
50 ml |
250 ml |
| Buffer RW2* |
5 ml |
20 ml |
2 x 50 ml |
| RNase Free Water |
1.8 ml |
10 ml |
30 ml |
Storage and Stability
The kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.
Experiment Data
Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)
Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .
Q3.Are you a manufacturer ?
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.
Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.
Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.
Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.
Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.
Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.
Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.
Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.
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