HiPure Tissue&Blood DNA Maxi Kit
| CAT NO |
PRODUCT NAME |
SIZE |
PRICE |
| D311502 |
HiPure Tissue&Blood DNA Maxi Kit |
10 preps |
$174.00 |
| D311503 |
HiPure Tissue&Blood DNA Maxi Kit |
50 preps |
$622.00 |
CAT NOPRODUCT NAMESIZE
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Specifications
| Features |
Specifications |
| Main Functions |
Isolation total DNA from 10ml blood and 1g tissue using Maxi column |
| Applications |
PCR, southern bolt and virus detection, etc |
| Purification method |
Maxi spin column |
| Purification technology |
Silica technology |
| Process method |
Manual (centrifugation or vacuum) |
| Sample type |
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount |
3-10ml |
| Elution volume |
≥700μl |
| Time per run |
≤90 minutes |
| Liquid carrying volume per column |
4ml |
| Binding yield of column |
5mg |
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
- High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
- Fast - without separation of leukocytes, organic extraction or ethanol precipitation
- Simple - all nucleic acids can be obtained by direct digestion
- Pertinence - specially designed for isolating DNA from 3-10ml blood and related body fluids
- Wide applicability - handle a variety of liquid samples
- Economy - excellent cost effectiveness performance
Kit Contents
| Contents |
D311502 |
D311503 |
| Purification Times |
10 |
50 |
| HiPure gDNA Maxi Columns |
10 |
50 |
| 50ml Collection Tubes |
20 |
100 |
| Buffer ATL |
120 ml |
550 ml |
| Buffer AL |
120 ml |
2 x 300 ml |
| Buffer GW1* |
53 ml |
220 ml |
| Buffer GW2* |
25 ml |
110 ml |
| RNase A |
40 mg |
180 ml |
| Proteinase K |
120 mg |
540 mg |
| Protease Dissolve Buffer |
12 ml |
50 ml |
| Buffer AE |
30 ml |
120 ml |
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Purchase guide
| Name |
CAT NO |
Sample amount |
Leukocyte protocol* |
Colum type |
Elutio volume |
Average yield |
Time per run |
| HiPure Blood DNA Mini Kit |
D3111 |
10-200μl |
1ml |
2ml column |
≥20μl |
5-9μg/200μl |
≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit |
D3113 |
0.2-2ml |
10ml |
1.5ml column |
≥300μl |
20-40μg/1m |
≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit |
D3115 |
3 -10ml |
10ml |
15ml column |
≥700μl |
20-40μg/1m |
≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit |
D3117 |
1-200μl |
1ml |
96 well plate |
|
3-8μg/200μl |
|
*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate,and white blood cells were obtained by centrifugation before extraction
Experiment Data
Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)
Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .
Q3.Are you a manufacturer ?
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.
Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.
Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.
Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.
Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.
Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.
Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.
Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.
Audited Supplier 
){kind=link}