HiPure Soil RNA Kit Isolation total RNA from 500 mg soil sample

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: R418302 / R418303
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Isolation total RNA from 500 mg soil sample
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, RT-PCR, Northern blot, poly-A + purification
• Source: Lab
• Habit Appellation: HiPure Soil RNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Main Functions: Isolation total RNA from 500 mg soil sample
• Applications1: RT-PCR, Northern blot, poly-A + purification
• Applications2: nucleic acid protection in vitro translation
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type1: Forest soil, grassland soil
• Sample Type2: mining area soil, sediment and other samples
• Sample Amount: 500 mg
• Elution Volume: ≥30μl
• Time per run: ≤60 minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Transport Package: Bottle/ Box
• Specification: 50 preps / 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822190090
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure Soil RNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
R418302	HiPure Soil RNA Kit	50 preps	$298.00
R418303	HiPure Soil RNA Kit	250 preps	$1,343.00

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.



Specifications
 

Features	Specifications
Main Functions	Isolation total RNA from 500 mg soil sample
Applications	RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Forest soil, grassland soil, mining area soil, sediment and other samples
Sample amount	500 mg
Elution volume	≥30μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

 

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.


Advantages
High quality - high purity total RNA can be directly used in various sensitive downstream applications
Fast - extraction of several samples can be completed in 60 minutes by column purification method
Safe - no phenol chloroform extraction required
Sensitive - RNA can be purified at the level of PG


Kit Contents

Contents	R418302	R418303
Purification Times	50 Preps	250 Preps
HiPure RNA Micro Columns	50	250
2ml Collection Tubes	100	500
gDNA Filter Column	50	250
2ml Beads Tubes	50	250
Buffer SOL	30 ml	150 ml
Buffer SDS	4 ml	15 ml
Buffer PHC	30 ml	150 ml
Buffer GDP	40 ml	150 ml
Buffer RW1	50 ml	200 ml
Buffer RW2 *	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability
Buffer PHC should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under theseconditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data


 

Detailed Photos

 

Protocol

 

 

Company Profile

 

 

FAQ

 

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer ? 
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.

Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.

Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.

Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.