Recover DNA fragments HiPure DNA Clean Up Kit

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D214102 / D214103
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Recover DNA fragments between 20bp-20kbp
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, sequencing, labeling reactions,
• Source: Lab
• Habit Appellation: HiPure DNA Clean Up Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Main Functions: Recover DNA fragments between 20bp-20kbp
• Applications1: PCR, sequencing, labeling reactions,
• Applications2: ligations and restriction digestion
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type1: Crude DNA, enzymatic reaction solution
• Sample Amount: <150μl
• Recovery: 80%
• Elution Volume: ≥15μl
• Time per run: ≤10 minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 20µg
• Transport Package: Bottle/ Box
• Specification: 100 preps / 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822190090
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure DNA Clean Up Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
D214102	HiPure DNA Clean Up Kit	100 preps	$60.00
D214103	HiPure DNA Clean Up Kit	250 preps	$150.00

HiPure DNA Clean Up Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.



Specifications
 

Features	Specifications
Main Functions	Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Crude DNA, enzymatic reaction solution
Sample amount	<150μl
Recovery	80%
Elution volume	≥15μl
Time per run	≤10 minutes
Liquid carrying volume per column	800µl
Binding yield of column	20µg

 

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.


Advantages
High recovery efficiency - up to 80% DNA recovery
Fast - isolation can be completed in 10 minutes by column gel method
Low elution volume - elution volume can be as low as 10μl
Efficient - efficient removal of various inhibitory factors


Kit Contents

Contents	D214102	D214103
Purification Times	100 Preps	250 Preps
Buffer GWP	30 ml	60 ml
Buffer DW2*	20 ml	50 ml
Elution Buffer	30 ml	60 ml
HiPure DNA Mini Columns I	100	250
2 ml Collection Tubes	100	2 x 125

Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve.

 

Detailed Photos

Protocol

 

Company Profile

 

 

FAQ

 

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer ? 
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.

Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.

Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.

Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.