HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp

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Year of Establishment
2021-01-29
Number of Employees
4
  • HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
  • HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
  • HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
  • HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
  • HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
  • HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
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Overview

Basic Info.

Model NO.
D212201 / D212202 / D212203
EINECS
/
Classification
Biochemical Reagents
Grade
Reagent Grade
Specific Usage
For Biological Purpose, For Microbiological, PCR, Sequencing, Labeling Reactions
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Recover DNA fragments between 60bp-10kbp from PCR
Source
for Lab Research
Habit Appellation
HiPure Clean Up 96 Kit
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Ligations and Restriction Digestion
Property
Biochemical Reagent
Main Functions
Recover DNA fragments >100bp from various pcr
Main Functions2
enzymatic reaction solutions, labeling reaction so
Applications1
PCR, Sequencing, Labeling Reactions
Applications2
Ligations and Restriction Digestion
Purification Method1
Mini Spin Column
Purification Technology
Silica Technology
Process Method
Manual (Centrifugation or Vacuum)
Sample Type1
PCR Products, Enzymatic Reaction Solution
Sample Amount1
10-200μl
Recovery
≥80%
Elution Volume
≥15μl
Time Per Run
≤10 Minutes
Liquid Carrying Volume Per Column
800µl
Binding yield of column
35µg
Sample
Free Sample
Transport Package
Box
Specification
100 preps / 250 preps
Trademark
Magen Biotech
Origin
China

Product Description

Product Description

HiPure Clean Up 96 Kit
Recover DNA fragments between 60bp-10kbp from PCR, reactions, crude DNA using 96 well bind plate

CAT NO PRODUCT NAME SIZE PRICE
D212201 HiPure Clean Up 96 Kit 1 x 96 preps $152.00
D212202 HiPure Clean Up 96 Kit 4 x 96 preps $300.00
D212203 HiPure Clean Up 96 Kit 20 96 preps $1,400.00





Introduction

HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.




Details

Features Specifications
Main Functions Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate
Applications PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method 96 well Bind Plate
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type PCR product, enzymatic reaction
Sample amount Appropriate
Recovery ≥80%
Elution volume 75μl
Time per run ≤60 minutes
Liquid carrying volume per column 800µl
Binding yield of column 20µg



Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

  • High recovery efficiency - 80% DNA recovery
  • Fast - isolation can be completed in 60 minutes





Kit Contents

Contents D212201 D212202 D212203
Purification Times 1 x 96 Preps 4 x 96 Preps 20 x 96 Preps
Buffer GDP 120 ml 400 ml 4 x 400 ml
Buffer DW2 20 ml 100 ml 3 x 100 ml
Elution Buffer 20 ml 20 ml 30 ml
HiPure DNA Plate 1 4 20
2.2ml Collection Plate 1 4 20
1.6 ml Collection Plate 1 4 20
0.8 ml Collection Plate 1 4 20




Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
If any precipitates form in the buffers, warm at 37ºC to dissolve.





 

Protocol

 

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp


 

Company Profile

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp

 

Exhibition all over the world

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp



 

Packaging & Shipping

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp





 

Certificate

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbpHiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp

 

Laboratory

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp
 

Workshop

HiPure Clean Up 96 Kit Recover DNA fragments between 60bp-10kbp

 

 

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