Hipure PCR Pure Mini Kit Recover DNA Fragments Between 60bp-20kbp

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 100 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: D212102
• HS Code: 3822009090

Product Description

Product Description

Product Name HiPure PCR Pure Mini Kit
Cat. No. & Specifications D212102,100Preps/kit
Introduction
HiPure PCR Pure Mini Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations,PCR,sequencing,restriction digestion,or various labeling reactions.In addition,this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.



Main Composition

Product	D2121-02
Purification Times	100 Preps
Buffer DP	60 ml
Buffer DW2	20 ml
Elution Buffer	10 ml
HiPure DNA Mini Columns I	100
2 ml Collection Tubes	100

Storage conditions and Validity
HiPure Gel Pure DNA Kits components are guaranteed for at least one year when stored at room temperature.If any precipitates form in the buffers,warm at 37ºC to dissolve.
  

Materials and Equipment to be Supplied by User

1.Dilute Buffer DW2 with 100% ethanol and store at room temperature.

2.Microcentrifuge capable of at least 13,000 × g

Troubleshooting Guide
1.  Low or no recovery

Buffer DW2 did not contain ethanol:  Ethanol must be added to Buffer DW2 before used. Repeat precedweure with correctly prepare Buffer PE.

Inappropriate Elution Buffer: DNA will only be eluted efficiently in the presende of low salt buffer or Water.

Sample volume too high or low: for reaction cleanup, The sample volume must be in the range of 20~200ul.

2.  DNA does not perform well (e.g. in ligation reaction)

Salt concentration in eluate too high: Modify the wash step by incubating the column for 5 min at room temperature after adding 650ul of Buffer DW2, then centriufge.

Eluate contains residual ethanol: Ensure that the wash flow-through is drained from the collection tube and that the column is then centrifuged at >12,000 x g for 1min.  

Eluate contaminated with Primer-dimers: Primer-dimers are >20bp, and are not completely removed. After the binding step, wash the Column with 700ul of a 35% guanidine hydrochloride solution. Continue with the Buffer DW2 wash step.
 

Eluate contains denatured ssDNA, which appears as smaller smeared band on analitical gel: Use the Eluted DNA to prepare the subsequent enzymatic reaction but omit the enzyme. To reanneal the ssDNA, incubate the reaction mixture at 95oC for 2 min, and allow the tube to cool slowly to room temperature. Add enzyme and proceed as usual.

Packaging & Shipping

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