HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp

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Number of Employees
4
Year of Establishment
2021-01-29
  • HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
  • HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
  • HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
  • HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
  • HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
  • HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
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Overview

Basic Info.

Model NO.
D212002 / D212003
EINECS
/
Classification
Biochemical Reagents
Grade
Reagent Grade
Specific Usage
For Biological Purpose, PCR, Sequencing, Labeling Reactions
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Recover DNA Fragments PCR Products
Source
for Lab Research
Habit Appellation
HiPure PCR Pure Micro Kit
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Ligations and Restriction Digestion
Property
Biochemical Reagent
Main Functions
Recover DNA fragments between 60bp-20kbp from PCR
Main Functions2
enzymatic reaction solution
Applications1
PCR, Sequencing, Labeling Reactions
Applications2
Ligations and Restriction Digestion
Purification Method1
Mini Spin Column
Purification Technology
Silica Technology
Process Method
Manual (Centrifugation or Vacuum)
Sample Type1
PCR Products, Enzymatic Reaction Solution
Sample Amount1
10-200μl
Recovery
≥80%
Elution Volume
≥7μl
Time Per Run
≤10 Minutes
Liquid Carrying Volume Per Column
800µl
Binding yield of column
10µg
Sample
Free Sample
Transport Package
Box
Specification
100 preps / 250 preps
Trademark
Magen Biotech
Origin
China

Product Description

Product Description

HiPure PCR Pure Micro Kit
Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution
 

CAT NO PRODUCT NAME SIZE PRICE
D212002 HiPure PCR Pure Micro Kit 100 preps $60.00
D212003 HiPure PCR Pure Micro Kit 250 preps $150.00



Introduction

HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.




Details
 

Features Specifications
Main Functions Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution
Applications PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type PCR products, enzymatic reaction solution
Sample amount 10-200μl
Recovery 80%
Elution volume l
Time per run ≤10 minutes
Liquid carrying volume per column 800µl
Binding yield of column 10µg



Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.


Advantages
  • High recovery efficiency - 80% DNA recovery
  • Fast - isolation can be completed in 10 minutes by column gel method





Kit Contents

Contents D212002 D212003
Purification Times 100 Preps 250 Preps
Buffer DP 60 ml 120 ml
Buffer DW2 20 ml 50 ml
Elution Buffer 15 ml 30 ml
HiPure DNA Micro Columns 100 250
2 ml Collection Tubes 100 250




Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
If any precipitates form in the buffers, warm at 37ºC to dissolve.




 

Protocol

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp




 

Company Profile

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp

 

Exhibition all over the world

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp



 

Packaging & Shipping

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp



 

Certificate

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbpHiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp

 

Laboratory

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp
 

Workshop

HiPure PCR Pure Micro Kit Recover DNA fragments between 60bp-20kbp

 

 

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