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MagPure Bacterial DNA Kit
CAT NO | PRODUCT NAME | SIZE | PRICE |
D513101 | MagPure Bacterial DNA Kit | 48 preps | $174.00 |
D513102 | MagPure Bacterial DNA Kit | 96 preps | $298.00 |
D513103 | MagPure Bacterial DNA Kit | 480 preps | $1,293.00 |
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from bacterial culture |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Gram negative or positive bacteria |
Sample amount | Bacterial culture: 0.5-1.5ml |
Elution volume | ≥60μl |
Time per run | ≤60 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Kit Contents
Contents | D513101 | D513102 | D513103 |
Purification Times | 48 Preps | 96 Preps | 480 Preps |
MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
Lysozyme | 60 mg | 120 mg | 600 mg |
Proteinase K | 24 mg | 50 mg | 220 mg |
Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
Buffer SDS | 1.5 ml | 3 ml | 15 ml |
Buffer MLA | 30 ml | 60 ml | 300 ml |
Buffer GW1* | 13 ml | 44 ml | 110 ml |
Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)
Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .
Q3.Are you a manufacturer ?
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.
Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.
Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.
Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.
Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.
Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.
Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.
Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.