Premium Hipure Yeast DNA Isolation Kit for Cultures

Product Details
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Number of Employees
4
Year of Establishment
2021-01-29
  • Premium Hipure Yeast DNA Isolation Kit for Cultures
  • Premium Hipure Yeast DNA Isolation Kit for Cultures
  • Premium Hipure Yeast DNA Isolation Kit for Cultures
  • Premium Hipure Yeast DNA Isolation Kit for Cultures
  • Premium Hipure Yeast DNA Isolation Kit for Cultures
  • Premium Hipure Yeast DNA Isolation Kit for Cultures
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  • Overview
  • Product Description
  • Protocol
  • Company Profile
  • FAQ
Overview

Basic Info.

Model NO.
D314702 / D314703
EINECS
/
Classification
Biochemical Reagents
Grade
Reagent Grade
Specific Usage
For Biological Purpose, For Microbiological, DNA Isolation From Yeast Samples
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, Southern Blot, Enzyme Digestion and Ngs
Source
Lab Production
Habit Appellation
Hipure Yeast DNA Kit
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, PCR, Southern Blot and Enzyme Digestion
Property
Biochemical Reagent
Main Functions
Isolation Total DNA From Yeast Cultures
Applications1
PCR, Southern Blot and Enzyme Digestion, etc.
Purification Method
Mini Spin Column
Purification Technology
Silica Technology
Process Method
Manual (Centrifugation or Vacuum)
Sample Type
Yeast Culture
Sample Amount
Bacterial Culture: 1-1.5 Ml
Elution Volume
≥30μl
Time Per Run
≤60 Minutes
Liquid Carrying Volume Per Column
800μl
Binding Yield of Column
100μg
Total DNA
Yeast (<5X 107)
Sample
Free Sample
Transport Package
Bottle/ Box
Specification
50 preps / 250 preps
Trademark
Magen Biotech
Origin
China
Production Capacity
100000 Per Month

Product Description

Product Description

HiPure Yeast DNA Kit
Isolation DNA from yeast culture (<5x 10^7)

CAT NO PRODUCT NAME SIZE PRICE
D314702 HiPure Yeast DNA Kit 50 preps $154.00
D314703 HiPure Yeast DNA Kit 250 preps $694.00




Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.




Details
Features Specifications
Main Functions Isolation total DNA from yeast cultures
Applications PCR, southern blot and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Yeast culture
Sample amount

Bacterial culture: 1-1.5 ml

Elution volume ≥30μl
Time per run ≤60 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg



Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).






Advantages
  • Fast - several samples can be extracted in 40 minutes (after digestion)
  • High purity - purified DNA can be directly used in various downstream applications
  • Good repeatability - silica technology can obtain ideal results every time
  • High recovery - DNA can be recovered at the level of PG
  • Sufficient components - lysozyme, protease K, RNase A and glass beads



Kit Contents
Contents D314702 D314703
Purification Times 50 Preps 250 Preps
Hipure DNA Mini Columns I 50 250
2ml Collection Tubes 50 250
Glass Beads (0.4~0.6mm) 20 g 90 g
Buffer SE 30 ml 150 ml
Lyticase 1.8 ml 5 x 1.8 ml
Buffer ATL 30 ml 150 ml
ReagentDX 500 μl 1500 μl
Buffer DL 30 ml 150 ml
Buffer GW1* 13 ml 66 ml
Buffer GW2* 20 ml 2 x 50 ml
Proteinase K 12 mg 60 mg
Protease Dissolve Buffer 1.8 ml 5 ml
Buffer AE 15 ml 30 ml







Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37ºC water bath.


 

Protocol

Premium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for Cultures




 

Company Profile

 

Premium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for CulturesPremium Hipure Yeast DNA Isolation Kit for Cultures

FAQ

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.


Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.


Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.


Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.

 



 

 

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