Hipure Plasmid Ef MIDI Kit (Spin) Plasmid DNA

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• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, Cell Transfection, Animal Injection
• Model NO.: P123102B
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation up to 10mg Endotoxin-Free Plasmid DNA
• Source: for Lab Research
• Habit Appellation: Hipure Plasmid Ef MIDI Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Cell Transfection, Animal Injection, etc.
• Property: Biochemical Reagent
• Main Functions: Isolation 500µg Endotoxin-Free Plasmid DNA
• Applications1: Cell Transfection, Animal Injection, etc.
• Purification Method1: MIDI Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type1: Conventional Plasmid, Plasmid Less Than 30kb
• Sample Amount1: 25-50ml
• Yield: 10-500μg
• Elution Volume: ≥0.4ml
• Time Per Run: ≤60 Minutes
• Liquid Carrying Volume Per Column: 4ml
• Binding Yield of Column: 250μg
• After-Sales Service: Technical Support
• Sample: Free Sample
• Shipping: FedEx, DHL, Door to Door
• Transport Package: Box
• Specification: 50 preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

HiPure Plasmid EF Midi Kit (Spin)

CAT NO	PRODUCT NAME	SIZE	PRICE
P123102	HiPure Plasmid EF Midi Kit(Vacuum)	50 preps	$423.00
P123102B	HiPure Plasmid EF Midi Kit(Spin)	50 preps	$547.00

△ Introduction

The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.

Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

△ Details

 

Features	Specifications
Main Functions	Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture
Applications	Cell transfection, animal injection, etc.
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	25-50ml
Yield	10-500μg
Elution volume	≥0.4ml
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	250μg

△  Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - silica gel column purification is much faster than ion exchange
• High yield - up to 250μg plasmid can be binded in one column
• Low endotoxin content - the obtained plasmid can be directly used in cell transfection and animal injection

△ Kit Contents

Contents	P123102	P123102B
Purification Times	50 Preps	50 Preps
RNase A	30 mg	30 mg
Buffer E1	150 ml	150 ml
Buffer E2	150 ml	150 ml
Buffer E3	150 ml	150 ml
Buffer E4	150 ml	150 ml
Buffer E5	150 ml	150 ml
Buffer PW2*	50 ml	50 ml
Elution Buffer	30 ml	30 ml
MaxPure EF Mini Column	50	50
2 ml Collection Tubes	50	50
Lysate Clear Midi Syringe	50	50
Extend Tubes	50	50
50ml Centrifuge Tubes		50
Support Tubes		50

△ Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C.

 

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