Hipure Universal Mirna Kit Small Rna Molecules (18nt)

• CAS No.: /
• Formula: /
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, Nucleic Acid Protection and in Vitro Translation
• Model NO.: R431002/R431003
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolate Mirna Small Rna Molecules
• Source: for Lab Research
• Habit Appellation: Hipure Universal Mirna Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Rt-PCR, Northern Blot, Poly a+Purification
• Property: Biochemical Reagent
• Main Functions: Isolation Mirna and Other Small Rna Molecules 18nt
• Applications1: Rt-PCR, Northern Blot, Poly a+Purification
• Applications2: Ucleic Acid Protection and in Vitro Translation
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Animal Tissues, Adherent Cells, Suspension Cells
• Sample Amount1: Eukaryotic Culture Cells: ≤ 10^7
• Sample Amount2: Animal Tissue:<100mg, Yeast Culture Cells:<5 X10^7
• Sample Amount3: Bacteria:<10^9
• Elution Volume: ≥15μl
• Time Per Run: ≤40 Minutes
• Sample: Free Sample for Test
• After-Sales Service: Technical Support
• Shipping: FedEx, DHL, Door to Door
• Transport Package: Box
• Specification: 50 Preps/ 250Preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

HiPure Universal miRNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
R431002	HiPure Universal miRNA Kit	50 preps	$174.00
R431003	HiPure Universal miRNA Kit	250 preps	$746.00

△ Introduction

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately.

This Kit combines phenol/guanidine-based lysis of samples and silica membrane-based purification of total RNA.MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
 

△ Details

Features	Specifications
Main Functions	Isolation miRNA and other small RNA molecules(18nt), from cultured cells and various animal and human tissues, using MagZol reagent and column
Applications	RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissues, adherent cells, suspension cells, bacteria, etc
Sample amount	Eukaryotic culture cells: ≤ 10^7, Animal tissue:<100mg, Yeast culture cells:<5 x10^7, Bacteria:<10^9
Elution volume	≥15μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

△  Advantages

• High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast - several samples can be extracted in 40 minutes
• High applicability - samples including animals, plants, bacteria, cells, etc.
• High concentration - efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity

△ Kit Contents
 

Contents	R431002	R431003
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	100	2 x 250
2ml Collection Tubes	100	2 x 250
MagZol Reagent	60 ml	270 ml
Buffer RWC	20 ml	80 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

 

 

△ Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.



 

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