P115402 Hipure Plasmid Ef Mini Kit with Rnase a

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: P115402

Product Description

Product Description

Introduction
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen's innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments.


The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.






Specifications

Features	Specifications
Main Functions	Isolation up to 70µg endotoxin-free plasmid DNA from 10-15ml bacterial culture
Applications	Cell transfection, animal injection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid
Sample amount	High copy plasmid: 10ml culture medium Low copy number plasmid : 10-15ml culture medium
Yield	10-70μg
Elution volume	≥75μl
Time per run	≤40 minutes
Liquid carrying volume per column	800μl
Binding yield of column	70μg

Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).





Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - silica gel column purification is much faster than ion exchange
• High yield - up to 70μg plasmid can be binded in one column
• Low endotoxin content - the obtained plasmid can be directly used in cell transfection and animal injection

Kit Contents

Product Number	P115402	P115403
Purification Times	50 Preps	250 Preps
RNase A	5 mg	20 mg
Buffer P1	30 ml	140 ml
Buffer P2	30 ml	140 ml
Buffer LEN3	15 ml	70 ml
Buffer LN4	50 ml	250 ml
Buffer LN5	30 ml	140 ml
Buffer PW1	30 ml	140 ml
Buffer PW2	12 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns III	50	250
2 ml Collection Tubes	50	250

Storage conditions and Validity
HiPure Plasmid EF Mini Kits components are guaranteed for at least one year when stored at room temperature.
If any precipitates form in the buffers,warm at 37ºC to dissolve.
After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C.






Materials and Equipment to be Supplied by User

1.Dilute Buffer PW2 with 100% ethanol and store at room temperature

2.Add the vial of RNase A to the bottle of Buffer P1 and store at 2-8˚C

3.Heat Elution Buffer to 70°C if plasmid DNA is >10kb

4.HiPure DNA Mini Column III can bind up to 70µg DNA.

Packaging & Shipping

Certificate

Laboratory

Workshop

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