Hipure PCR Pure Mini Kit PCR Use Directly

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 100 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: D212102

Product Description

Product Description

Introduction
HiPure PCR Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.





Specifications

Features	Specifications
Main Functions	Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR products, enzymatic reaction solution
Sample amount	10-200μl
Recovery	≥80%
Elution volume	≥15μl
Time per run	≤10 minutes
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.




Advantages

• High recovery efficiency - ≥80% DNA recovery
• Fast - isolation can be completed in 10 minutes by column gel method

Kit Contents

Contents	D212102	D212103
Purification Times	100 Preps	250 Preps
Buffer DP	60 ml	120 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve.





Experiment Data

 

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