Hipure Stool DNA Kit Total DNA

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D314102 / D314103
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Isolation Total DNA From 50-100mg Stool Sample
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, Southern Blot, Enzyme Digestion and Ngs
• Source: Lab Production
• Habit Appellation: Hipure Stool DNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Obtain High-Quality DNA
• Property: Biochemical Reagent
• Main Functions: Isolation Total DNA From 50-100mg Stool Sample
• Applications1: PCR, Southern Blot, Enzyme Digestion and Ngs
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Stool
• Stool Samples1: Gut Microbial DNA / Food Residue Sample DNA
• Stool Samples2: Alimentary Tract Exfoliated Cell DNA.
• Sample Amount: 50-100mg
• Yield: 3-15μg
• Elution Volume: ≥30μl
• Time Per Run: ≤60 Minutes
• Liquid Carrying Volume Per Column: 750μl
• Binding Yield of Column: 100μg
• Sample: Free Sample
• Transport Package: Bottle/ Box
• Specification: 50 preps/ 250 preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

HiPure Stool DNA Kit
Isolation total DNA from 50-100mg stool samples

CAT NO	PRODUCT NAME	SIZE	PRICE
D314102	HiPure Stool DNA Kit	50 preps	$174.00
D314103	HiPure Stool DNA Kit	250 preps	$746.00

 

△ Introduction

With the development of molecular biology, stool, a new non-invasive sample, has been widely used in the research of animal molecular genetics, population ecology, behavioral ecology and some intestinal disease diagnosis. Stool samples includes gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.



The primary problem encountered when using stool sample for molecular biology research is the low content of exfoliated cells in the digestive tract and a certain degree of degradation of genetic material in stool. Another issue in molecular scatology research based on PCR is the presence of a large number of inhibitors in stool that can affect Taq enzyme activity, leading to downstream detection inactivation. These inhibitors include polysaccharides, plant polysaccharides, bile acids, bile salts, bile pigments, digestive juices, mucus, etc. Therefore, selecting appropriate extraction methods to obtain high-quality DNA is the key to successful downstream detection of stool DNA.



At present, the pretreatment methods used in the laboratory, such as phenol/chloroform extraction, cetyltrimethyl bromide (CTAB) lysis, and guanidine isothiocyanate lysis, lack universality in different species, and the success rate of extracting DNA for PCR amplification is also very low. The HiPure Stool DNA Kit provided by Magen Company has opened up a new approach for DNA extraction from stool samples with good universality, high cost-effectiveness, high yield and purification. The reagent kit adopts a unique solution system and inhibitory factor adsorbent, which can efficiently remove various impurities in stool samples. The purified DNA can be directly used for PCR, quantitative PCR and other applications.




This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.





△ Details

Features	Specifications
Main Functions	Isolation total DNA from 50-100mg stool samples
Applications	PCR, Southern Blot, enzyme digestion and NGS, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Stool
Sample amount	50-100mg
Yield	3-15μg
Elution volume	≥30μl
Time per run	≤60 minutes
Liquid carrying volume per column	750μl
Binding yield of column	100μg

△  Advantages

• High purity - unique adsorbent can completely remove inhibitory factors
• High concentration - maximum extraction of total DNA from stool samples
• High recovery - DNA can be recovered at the level of PG
• Good repeatability - silica technology can obtain ideal results every time

△ Kit Contents

Contents	D314102	D314103
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Columns II	50	250
2ml Collection Tubes	50	250
2ml Bead Tubes	50	250
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer SPL	40 ml	200 ml
Buffer PCI	40 ml	200 ml
Buffer AL	20 ml	80 ml
Buffer GW1	22 ml	88 ml
Buffer GW2	20 ml	2 x 50  ml
Buffer AE	15 ml	30 ml

△ Storage and Stability

Proteinase K and Buffer PCI should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

 

 

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