Hipure Ffpe Rna Kit Slice Ffpe Embedded Tissue

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• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, for Tumor Detection Cancer Screening
• Model NO.: IVD4144
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation Total Rna From Ffpe Tissue
• Source: Lab Production
• Habit Appellation: Hipure Ffpe Rna Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Tumor Detection
• Property: Biochemical Reagent
• Main Functions: Isolation Total Rnafrom Ffpe Tissue/Section Sample
• Applications1: Rt-PCR, Quantitative Rt-PCR, Northern Hybridizatio
• Applications2: Poly a Purification, Nucleic Acid Protection
• Applications3: in Vitro Translation
• Products: Tumor Testing, Cancer Screening, Rna, Mirna
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology, Dnase
• Process Method: Manual (Centrifugation or Vacuum)
• Adaptive Instrument: Nucleic Acid Extractor, Pipetting Workstation
• Sample Type: Ffpe Slice, Ffpe Embedded Tissue
• Sample Amount1: Tissue: <6 Mg; Ffpe: <6 Slices
• Yield: 1-20μg
• Elution Volume: ≥15μl
• Time Per Run: ≤30 Minutes (After Digestion)
• Liquid Carrying Volume Per Column: 800μl
• Transport Package: Bottle/ Box
• Specification: 50 preps/ 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

 

Product Description

HiPure FFPE RNA Kit

 

CAT NO	PRODUCT NAME	SIZE	PRICE
IVD4144	HiPure FFPE RNA Kit	50 preps	$298.00

△ Introduction

HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.







△ Details

Features	Specifications
Main Functions	Isolation total RNA from FFPE tissue
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Products	RNA, miRNA
Purification method	Mini spin column
Purification technology	Silica technology, DNase
Process method	Manual (centrifugation or vacuum)
Sample type	FFPE slice, FFPE embedded tissue
Sample amount	Tissue: <6 mg FFPE: <6 slices
Yield	1-20μg
Elution volume	≥15μl
Time per run	≤30 minutes (after digestion)
Liquid carrying volume per column	800μl

△  Advantages

• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 60 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG

△ Kit Contents

Contents	IVD4144
Purification Times	50 Preps
HiPure RNA Micro Columns	50
2ml Collection Tubes	50
Proteinase K	24 mg
Protease Dissolve Buffer	1.8 ml
DNase I	600 μl
DNase Booster Buffer	1.5 ml
Buffer DPS	60 ml
Buffer FRL	15 ml
Buffer RLC	15 ml
Buffer RWC*	10 ml
Buffer RW2*	20 ml
RNase Free Water	10 ml

△ Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.







 

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