| CAS No.: | / |
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| Formula: | / |
| EINECS: | / |
| Classification: | Biochemical Reagents |
| Grade: | Reagent Grade |
| Specific Usage: | For Biological Purpose, For Microbiological, For Microscopic Purpose, Rt-PCR, Northern Blot, Poly a+Purification |
| Samples: |
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| Customization: |
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HiPure Cell miRNA Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R431102 | HiPure Cell miRNA Kit | 50 preps | $174.00 |
| R431103 | HiPure Cell miRNA Kit | 250 preps | $746.00 |
△ Introduction
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
△ Details
| Features | Specifications |
| Main Functions | Isolation total RNA and miRNA from cell and tissue without MagZol reagent |
| Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Cell, Animal tissue, Plant tissue |
| Sample amount | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
△ Advantages
| Contents | R431102 | R431103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer RLC | 40 ml | 200 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
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