Solpure Blood DNA Kit

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 50 ml
• Trademark: Magen Biotech
• Origin: China
• Model NO.: D331101
• HS Code: 90279000
• Production Capacity: 100000 Per Month

Product Description

Product Description

Introduction
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes.


The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.




Specifications

Features	Specifications
Main Functions	Isolation total DNA from whole blood using economic salt out method
Applications	PCR, enzyme digestion, Southern hybridization,etc.
Purification technology	Salting out method
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissue
Sample amount	Unlimited
Elution volume	≥100μl
Time per run	Variation with sample amount

Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation.


Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.




Advantages
 

• Safe - without phenol chloroform extraction
• Environment friendly - the reagents used are safe, non-toxic and without pollution
• High molecular weight - the molecular weight of genomic DNA is about 50-150kb
• High purity - the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
• Unlimited sample size - solution type operation, sample volume can be adjusted at will
• Cost performance - the most economical nucleic acid extraction technology

 

Kit Contents

Contents	D331101	D331102	D331103
Purification Times	50 ml	300 ml	1000 ml
10 x Buffer RBC	20 ml	100 ml	3 x 100 ml
Buffer WTL	60 ml	350 ml	1000 ml
Buffer PPS	20 ml	120 ml	350 ml
RNase Solution	300 µl	1.8 ml	6 ml
Buffer TE	10 ml	30 ml	120 ml

 

Storage and Stability

RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


 

 

Certifications

Packaging & Shipping

 

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