Hipure DNA Micro Kit

• Customization: Available
• Material: Plate,Liquid,Powder
• Transport Package: Box
• Model NO.: D312502
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.




Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-10μl blood, <2mg tissue and other samples
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissues, blood spots, swabs and various forensic samples
Sample amount	Blood:1-10μl, Tissue:<2mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).



 

Advantages

• Fast - several samples can be extracted in 20 minutes (after digestion)
• High purity - purified DNA can be directly used in various downstream applications
• High recovery - DNA can be recovered at the level of PG
• Good repeatability - silica technology can obtain ideal results every time

Kit Contents

Contents	D312502	D312503
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	60 ml
Buffer AL	15 ml	60 ml
Buffer GW1	22 ml	66 ml
Buffer GW2	20 ml	2 x 50 ml
Carrier RNA	310 μg	2 x 310 µg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml
HiPure DNA Mini Columns I	50	2 x 125
2 ml Collection Tubes	50	5 x 100

Storage and Stability
RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.




Experiment Data



 

Packaging & Shipping

Certificate

 

Laboratory

Workshop