Hipure Total Rna Plus Kit with Spin Column

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• Formula: /
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• Classification: Biochemical Reagents
• Grade: Classic
• Specific Usage: For Biological Purpose, For Microbiological, Isolation Total Rna (Not Include Mirna) From 20mg
• Model NO.: R411102/ R411103
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Teaching Reagents, Rt-PCR, Qrt-PCR, Northern Hybridization, Second Ge
• Source: Laboratory Production
• Habit Appellation: Hipure Total Rna Plus Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Rt-PCR, Qrt-PCR, Northern Hybridization, Second Ge
• Property: Biochemical Reagent
• Applications: Rt-PCR, Qrt-PCR, Northern Hybridization, Second Ge
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology, DNA Filtration Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Animal Soft Tissue, Cultured Cells, Lymphocytes, S
• Sample Amount1: Cells:≤1 X 107
• Sample Amount2: Animal Tissue Sample: 1-20 Mg
• Sample Amount3: Plant Tissue: 50-150 Mg
• Yield: 2-100μg
• Elution Volume: ≥50μl
• Time Per Run: ≤25 Minutes (1-24 Samples)
• Liquid Carrying Volume Per Column: 800µl
• Binding Yield of Column: 100µg
• Sample: Free Sample
• After-Sales Service: Technical Support
• Transport Package: Box
• Specification: 50 Preps/ 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 90279000
• Production Capacity: 100000 Per Month

Product Description

Product Description

 

HiPure Total RNA Plus Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
R411102	HiPure Total RNA Plus Kit	50 preps	$124.00
R411103	HiPure Total RNA Plus Kit	250 preps	$547.00

△ Introduction

This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.


△ Details
 

Features	Specifications
Main Functions	Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 10^6 cell using two columns (gDNA removed column)
Applications	RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method	Mini spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount	Cells:≤1 x 10^7 Animal tissue sample: 1-20 mg Plant tissue: 50-150 mg
Yield	2-100μg
Elution volume	≥50μl
Time per run	≤25 minutes(1-24 samples)
Liquid carrying volume per column	800µl
Binding yield of column	100µg

△ Advantages

• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 25 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG

△ Kit Contents

Contents	R411102	D411103
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Columns	50	250
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	100	2 x 250
Buffer RLC	50 ml	200 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	12 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

△ Storage and Stability

HiPure Kit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
 

 

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