Magpure Plasmid Ef DNA Kit

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• Classification: for Lab
• Grade: Classic
• Specific Usage: For Biological Purpose, for Lab Research
• Model NO.: P181402
• Content: Standard
• Usage: Laboratory Reagents
• Source: for Lab Research
• Habit Appellation: Chemical Reagent
• Application: for Lab Research
• Property: Neutral
• Transport Package: Box
• Specification: 100 Preps/Kit
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

Product Name MagPure Plasmid EF Mini Kit
Cat. No. & Specifications  P181402,100 Preps/Kit  
Introduction
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. Theis kit also be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.




Main Composition

Product Number	P181402	P181403
Purification Times	100 Preps	500 Preps
RNase A	10 mg	50 mg
Buffer P1	30 ml	150 ml
Buffer P2	30 ml	150 ml
Buffer LEN3	20 ml	80 ml
Buffer LN4	60 ml	300 ml
Buffer LN5	60 ml	300 ml
Buffer GW1	53 ml	352 ml
Bind Bead N	4.8 ml	24 ml

Storage conditions and Validity
MagPure Plasmid DNA Kits components are guaranteed for at least one year when stored at room temperature.
If any precipitates form in the buffers,warm at 37ºC to dissolve.
After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.




Materials and Equipment to be Supplied by User

1. Dilute Buffer PW2 with 100% ethanol and store at room temperature

2. Add 28ml (100Preps)130ml (500Preps) or 2 x 700 ml (5000 Preps)  to Bind beads N and store at room temperature.

3. Add the vial of RNase A to the bottle of Buffer P1 and store at 2-8˚C

Packaging & Shipping

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