HiPure SF Plant DNA 96 Kit Isolation DNA 50 mg simple plant

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D316702 / D316703
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Biological Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, For Microscopic Purpose, Isolation Total DNA From100 Mg Plant
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, SSR, Aflp, Rapd and Southern Blot
• Source: Lab Produced
• Habit Appellation: HiPure SF Plant DNA 96 Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Purify DNA From simple plant
• Property: Biochemical Reagent
• Main Functions: Isolation total DNA from 50mg simple plant
• Applications: PCR, SSR, Aflp, Rapd and Southern Blot, etc.
• Purification Method: 96 well plate
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Economic Plant Samples
• Sample Amount1: Fresh / Frozen Plant Samples: ≤50mg
• Sample Amount2: Gdried Plant / Seed Samples: ≤15mg
• Elution Volume: ≥75μl
• Time Per Run: 30-50 Minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Easy-to-Use: Purification Requires No Phenol or Chloroform Extr
• After-Sales Service: Technical Support
• Sample: Free Sample for Testing
• Transport Package: Box
• Specification: 4 x 96 preps / 20 x 96 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

 

Product Description

HiPure SF Plant DNA 96 Kit
Isolation total DNA from 50 mg simple plant using 96 well bind plate without chloroform

CAT NO	PRODUCT NAME	SIZE	PRICE
D316702	HiPure SF Plant DNA 96 Kit	4 x 96 preps	$796.00
D316703	HiPure SF Plant DNA 96 Kit	20 x 96 preps	$3,729.00

 

Introduction
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

△ Details

Features	Specifications
Main Functions	Isolation total DNA from 50mg simple plant using 96 well bind plate
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤50mg Dried plant / seed samples: ≤15mg
Elution volume	≥75μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

 

△ Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.



△ Advantages

• Safe - require no phenol or chloroform extraction
• Fast - several samples can be extracted in 30 minutes by silica technology
• High purity - high quality DNA, completely remove inhibitors
• High yield - silica technology can achieve the highest yield

Kit Contents

Contents	D316702	D316703
Purification Times	4 x 96 Preps	20 x 96 Preps
RNase Solution	5 ml	22 ml
Elution Buffer	120 ml	500 ml
Buffer SPL	200 ml	2 x 500 ml
Buffer PS	100 ml	400 ml
Buffer GW1	220 ml	5 x 220 ml
Buffer GW2	100 ml	3 x 100 ml
1.6ml Collection Plate	4	20
HiPure gDNA Plate	4	20
2.2ml Collection Plate(DW Plate)	8	40
0.8ml Collection Plate(DW Plate)	4	20
Sealing Film	20	100

△ Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

 

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 - 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 - 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 - 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 - 150μl	3-30μg

 

Protocol

 

 

Company Profile

 

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Packaging & Shipping

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