Hipure Sf Plant DNA Kit Isolation Total DNA From Plant Without Chloroform

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• Classification: Biochemical Reagents
• Grade: Biological Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, For Microscopic Purpose, Isolation Total DNA From100 Mg Plant
• Model NO.: D316402 / D316403
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, SSR, Aflp, Rapd and Southern Blot
• Source: Lab Produced
• Habit Appellation: Hipure Sf Plant DNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Purify DNA From Plant and Fungal Tissue
• Property: Biochemical Reagent
• Main Functions: Isolation Total DNA From100 Mg Simple Plant
• Applications: PCR, SSR, Aflp, Rapd and Southern Blot, etc.
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Economic Plant Samples
• Sample Amount1: Fresh / Frozen Plant Samples: ≤100mg
• Sample Amount2: Gdried Plant / Seed Samples: ≤20mg
• Elution Volume: ≥30μl
• Time Per Run: 30-50 Minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Easy-to-Use: Purification Requires No Phenol or Chloroform Extr
• After-Sales Service: Technical Support
• Sample: Free Sample for Testing
• Transport Package: Box
• Specification: 50preps / 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

 

Product Description

HiPure SF Plant DNA Kit
 

CAT NO	PRODUCT NAME	SIZE	PRICE
D316402	HiPure SF Plant DNA Kit	50 preps	$99.00
D316403	HiPure SF Plant DNA Kit	250 preps	$447.00

 

△ Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

△ Details
 

Features	Specifications
Main Functions	Isolation total DNA from100 mg simple plant
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤100mg gDried plant / seed samples: ≤20mg
Elution volume	≥30μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

△ Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.



△ Advantages

• Safe - require no phenol or chloroform extraction
• Fast - several samples can be extracted in 30 minutes by silica technology
• High purity - high quality DNA, completely remove inhibitors
• High yield - silica technology can achieve the highest yield

△ Kit Contents

Contents	D316402	D316403
Purification Times	50 Preps	250 Preps
RNase A	10 mg	50 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer SPL	30 ml	150 ml
Buffer PS	10 ml	50 ml
Buffer GW1	22 ml	110 ml
Buffer GW2*	12 ml	2 x 50 ml
Buffer AE	15 ml	60 ml
HiPure gDNA Mini Columns	50	2 x 125
2 ml Collection Tubes	50	2 x 125

△ Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

△ Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 - 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 - 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 - 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 - 150μl	3-30μg

 

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