| CAS No.: | / |
|---|---|
| Formula: | / |
| EINECS: | / |
| Classification: | Biochemical Reagents |
| Grade: | Reagent Grade |
| Specific Usage: | For Biological Purpose, Purification of High Purity DNA From Bone Samples |
| Samples: |
|---|
| Customization: |
|---|
Suppliers with verified business licenses
Audited Supplier HiPure Bone DNA Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D312402 | HiPure Bone DNA Kit | 50 preps | $149.00 |
| D312403 | HiPure Bone DNA Kit | 250 preps | $696.00 |
△ Details
| Contents | D312402 | D312403 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer BGL | 40 ml | 180 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer GW1* | 13 ml | 53 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| DTT Powder | 235 mg | 2 x 235 mg |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Elution Buffer | 15 ml | 60 ml |
△ Storage and Stability
This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.
Bond grinding
The quality of STR maps from bone samples depends on the type of bone, age, and environmental storageconditions. Soil conditions and moisture have a profound effect on DNA quality. The successof theextraction process depends on the degree of grinding, which can be achieved by physical grindingor witha bit operated at a low speed to reduce heat build-up. The extraction process works best for fine-groundbone meal, where cells scattered throughout the bone matrix are easier to digest.
Bone meal grinder:
Pre-cool teeth or bones with liquid nitrogen, and pre-cool bone meal grinders withliquidnitrogen. Transfer the sample to the bone meal grinder, beat it hardly with a hammer several times, pre-coolthe grinder with liquid nitrogen, beat it several times until the sample forms a partial fine powder andsmallbone fragments, transfer the sample to the container, gently shake, and continue grinding thelargesampleinto powder. Gently oscillate in the container to pick out the fine powder for extraction process.
Bead mill: Please refer to the protocol of bead mill
Suppliers with verified business licenses
Audited Supplier 
){kind=link}
){kind=link}