HiPure Insect DNA Kit Isolation total DNA <10 mg insect tissue

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D312902 / D312903
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, For Microscopic Purpose, PCR, Southern Bolt and Virus Detection
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation total DNA from <10 mg insect tissue
• Source: Lab
• Habit Appellation: HiPure Insect DNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, PCR, Southern Bolt and Virus Detection, etc.
• Property: Biochemical Reagent
• Main Functions1: Isolation total DNA from <10 mg insect tissue
• Applications: PCR, southern bolt and virus detection, etc
• Purification Method: Midi Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Insect tissue samples
• Sample Amount: <10 mg
• Elution Volume: ≥15μl
• Time Per Run: ≤30 minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Sample: Free sample
• Transport Package: Box
• Specification: 50 Preps / 250 Preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

 

Product Description

HiPure Insect DNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
D312902	HiPure Insect DNA Kit	50 Preps	$175.00
D312903	HiPure Insect DNA Kit	250 Preps	$830.00

Introduction
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.





Details

Features	Specifications
Main Functions	Isolation total DNA from <10 mg insect tissue
Applications	PCR, southern bolt and virus detection, etc
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Insect tissue samples
Sample amount	<10 mg
Elution volume	≥15μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

 

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• • High purity DNA - can be used in sensitive downstream applications such as multiplex and quantitative pcr
  • Fast - several samples can be extracted in less than 30 minutes
  • Good repeatability - suitable for extracting high-yield DNA from insect tissue samples
  • Safe - without phenol chloroform extraction and alcohol precipitation

 Kit Contents

Contents	D312902	D312903
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Buffer ITL	30 ml	120 ml
Buffer IL*	30 ml	120 ml
Buffer GW1*	22 ml	110 ml
Buffer GW2*	20 ml	2 x 50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	15 ml
Buffer AE	15 ml	60 ml

 Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.



 

Protocol

 

 

Company Profile

Certifications

 

Detailed Photos

 

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