Hipure Fastfilter Plasmid MIDI Kit 250μ G Plasmid DNA

• CAS No.: /
• Formula: /
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• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, For Microscopic Purpose, For Scintillation, Isolation up to 250μg Plasmid DNA
• Model NO.: P101302/P101303
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Teaching Reagents, Cell Transfection, Animal Injection
• Source: Laboratory Production
• Habit Appellation: Hipure Fastfilter Plasmid MIDI Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Enzyme Digestion, Sequencing, PCR and Labeling
• Property: Biochemical Reagent
• Main Functions1: Isolation up to 250μg Plasmid DNA
• Main Functions2: From 30-50ml Bacterial Culture
• Applications1: Enzyme Digestion, Sequencing, PCR and Labeling,
• Purification Method: MIDI Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Conventional Plasmid, Plasmid Less Than 30kb
• Sample Amount1: 30-50ml
• Yield: 50-250µg
• Elution Volume: ≥300μl
• Time Per Run: ≤60 Minutes
• Liquid Carrying Volume Per Column: 4ml
• Binding Yield of Column: 250µg
• Sample: Free Sample
• After-Sales Service: Technical Support
• Transport Package: Box
• Specification: 25 Preps/100 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

Product Description

HiPure Fastfilter Plasmid Midi Kit
 

CAT NO	PRODUCT NAME	SIZE	PRICE
P101302	HiPure Fastfilter Plasmid Midi Kit	25 preps	$249.00
P101303	HiPure Fastfilter Plasmid Midi Kit	100 preps	$895.00

△ Introduction 

The HiPure Fastfilter Plasmid DNA Midi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes aspecial filter cartridge which replaces the centrifugation step following alkaline lysis.

Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 250 µg high copy number plasmid DNA or 10-75 µg low copy number plasmid DNA can be purified from 15-50 mL overnight culture.





△ Details

 

Features	Specifications
Main Functions	Isolation up to 250μg plasmid DNA from 30-50ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	30-50ml
Yield	50-250µg
Elution volume	≥300μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	250µg

△ Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - it takes only 15-60 minutes to complete the isolation
• High yield - up to 70µg plasmid can be binded in one column

△ Kit Contents
 

Contents	P101302	P101303
Purification Times	25 Preps	100 Preps
RNase A	10 mg	40 mg
Buffer P1	80 ml	300 ml
Buffer P2	80 ml	300 ml
Buffer LEN3	40 ml	150 ml
Buffer GBT	60 ml	250 ml
Buffer PW1	60 ml	250 ml
Buffer PW2	20 ml	100 ml
Elution Buffer	30 ml	120 ml
HiPure DNA Midi Columns III	25	100
Lysate Clear Midi Syringe	25	100
15 ml Collection Tubes	50	200

△ Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.



△ Purchase Guide

Name	CAT NO	Sample amount	Column  type	Elution  volume	Yield	Time per run	Centrifuge requirements
HiPure Plasmid Mini Kit	P1001	1-5ml	1.5ml column	30-50μl	5-35µg/5ml	≤30 minutes	small centrifuge
HiPure Plasmid Mini Kit II	P1002	5-15ml	1.5ml column	75-100μl	20-80µg/15ml	≤30 minutes	small centrifuge
HiPure Plasmid Plus 96 Kit	P1006	1-1.5ml	96 well plate	70-150μl	1-15μg/1ml	≤60 minutes	96 well plate centrifuge
HiPure Fastfilter Plasmid Midi Kit	P1013	30-50ml	15ml column	0.3-0.8ml	50-250µg/50ml	≤60 minutes	15ml centrifuge
HiPure Fastfilter Plasmid Maxi Kit	P1014	100-200ml	50ml column	0.5-1.5ml	0.4-1mg/200ml	≤60 minutes	50ml centrifuge

 

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