Product Description
MagPure Plasmid Mini Kit
CAT NO |
PRODUCT NAME |
SIZE |
PRICE |
P181102 |
MagPure Plasmid Mini Kit |
100 preps |
$59.00 |
P181103 |
MagPure Plasmid Mini Kit |
500 preps |
$239.00 |
P181104 |
MagPure Plasmid Mini Kit |
5000 preps |
$2,152.00 |
△
Introduction
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
△ Details
Features |
Specifications |
Main Functions |
Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture |
Applications |
Enzyme digestion, sequencing, PCR and labeling, etc. |
Purification technology |
Magnetic beads technology |
Process method |
Manual or automatic |
Sample type |
Conventional plasmid, plasmid≤30KB |
Sample amount |
1-3ml |
Elution volume |
≥50μl |
△ Advantages
- High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
- High yield - up to 20μg plasmid can be binded in one column
△ Kit Contents
Contents |
P181102 |
P181103 |
P181104 |
Purification Times |
100 Preps |
500 Preps |
5000 Preps |
RNase A |
10 mg |
50 mg |
2 x 250 mg |
Buffer P1 |
30 ml |
150 ml |
2 x 750 ml |
Buffer P2 |
30 ml |
150 ml |
2 x 750 ml |
Buffer N3 |
30 ml |
150 ml |
2 x 750 ml |
Buffer PW1 |
35 ml |
180 ml |
2 x 900 ml |
MagPure Particle NB* |
2.2 ml |
11 ml |
2 x 60 ml |
△ Storage and Stability
RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.
△ Experiment Data
Detailed Photos
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