Magpure Plasmid Mini Kit with Magnetic Beads

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• Formula: /
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• Classification: Biochemical Reagents
• Grade: Classic
• Specific Usage: For Biological Purpose, Isolation up to 20ug Plasmid DNA From 1-3ml Bacter
• Model NO.: P181102/ P181103/ P181104
• Content: Standard
• Usage: Laboratory Reagents
• Source: for Lab Research
• Habit Appellation: Magpure Plasmid Mini Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, PCR, Sequencing, Labeling Reactions, Ligations and
• Property: Biochemical Reagent
• Applications: Enzyme Digestion, Sequencing, PCR and Labeling
• Purification Technology: Magnetic Beads Technology
• Process Method: Manual or Automatic
• Sample Type: Conventional Plasmid, Plasmid≤30kb
• Sample Amount: 1-3ml
• Elution Volume: ≥50μl
• Sample: Free Sample for Test
• After-Sales Service: Technical Support
• Shipping: FedEx, DHL, Door to Door
• Transport Package: Box
• Specification: 100 Preps/ 500preps/ 5000preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

MagPure Plasmid Mini Kit
 

CAT NO	PRODUCT NAME	SIZE	PRICE
P181102	MagPure Plasmid Mini Kit	100 preps	$59.00
P181103	MagPure Plasmid Mini Kit	500 preps	$239.00
P181104	MagPure Plasmid Mini Kit	5000 preps	$2,152.00

△ Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.



△ Details

Features	Specifications
Main Functions	Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid≤30KB
Sample amount	1-3ml
Elution volume	≥50μl

△ Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• High yield - up to 20μg plasmid can be binded in one column

△  Kit Contents
 

Contents	P181102	P181103	P181104
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	50 mg	2 x 250 mg
Buffer P1	30 ml	150 ml	2 x 750 ml
Buffer P2	30 ml	150 ml	2 x 750 ml
Buffer N3	30 ml	150 ml	2 x 750 ml
Buffer PW1	35 ml	180 ml	2 x 900 ml
MagPure Particle NB*	2.2 ml	11 ml	2 x 60 ml

△ Storage and Stability

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.


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