Rnase a From Bovine Pancreas Enzyme Ribonuclease a

• CAS No.: 9001-99-4
• Formula: C9h14n4o3
• EINECS: 232-646-6
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Remove Rna From Preparations of Plasmid DNA
• Model NO.: C12120/ C12121
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Remove Rna Contamination of DNA and Protein Sample
• Source: Dry Powder
• Habit Appellation: Ribonuclease a (Rnase a), From Bovine Pancreas
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Remove Rna From Preparations of Plasmid DNA
• Property: Biochemical Reagent
• Appearance: White or Light Yellow Lyophilized Powder
• Molecular Weight: 13.7 Kda
• Purity: ≥60% Rnase a (SDS-Page)
• Specific Activity: >50 Kunitz Units/Mg Protein
• Preservation Conditions1: -20-8°c.
• Preservation Conditions2: Rnase Is Stable Under Heating and Deterge
• Dnase Residue Detection: Not Detected
• Application2: Remove Rna From Preparations of Plasmid DNA
• Application3: Hydrolyze Rna in Protein Samples
• Sample: Free Sample for Test
• After-Sales Service: Technical Support
• Shipping: FedEx, DHL, Door to Door
• Transport Package: Box
• Specification: 1g/10g
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

 

Product Description

RNase A
 

CAT NO	PRODUCT NAME	SIZE	PRICE
C12120	Rnase A	1 g	$300.00
C12121	Rnase A	10 g	$2,600.00

 

△ Introduction

RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphategroup attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2',3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. The highest activity is exhibited with single-stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.



△ Details

Features	Specifications
Appearance	White or light yellow lyophilized powder
Molecular weight	13.7 KDa
Purity	≥60% RNase A (SDS-PAGE)
Specific activity	>50 Kunitz units/mg protein
Preservation conditions	-20-8°C. RNase is stable under heating and detergent conditions and can withstand high temperature treatment of 100°C.
DNase residue detection	Not detected. In 10μl (including 200ng plasmid) solution, RNase A was added to the final concentration of 50-100 μg/ml and placed at room temperature for 30 minutes. After electrophoresis, the main band was obvious without degradation.
Application	Remove RNA from preparations of plasmid DNA

△ Advantages

• High activity, >50 U/mg
• High cost performance
• High purity, low impurity protein pollution
• No residual Dnase or trace Dnase

△ Ordering information

Contents	C12120	C12121
RNase A, Lyophilizate, >50 U/mg of protein	1 g	10 g

△ Storage and Stability
 Magen recommends storage at -20-8°C. When stored at 2-8°C, the product retains activity for at least 2 years.


 

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