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HiPure Total RNA Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| IVD4121 | HiPure Total RNA Kit | 50 preps | $348.00 |
△ Introduction
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
△ Details
| Features | Specifications |
| Main Functions | Isolation total RNA(miRNA)from tissue, cell using two columns and DNase plus reagent |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Products | RNA, miRNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Clinical tissues, cells, lymphocytes |
| Sample amount |
Tissue: <20 mg Cells: <5 x 106 |
| Yield | 2-50μg |
| Elution volume | ≥30μl |
| Time per run | ≤25 minutes |
△ Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
△ Advantages
△ Kit Contents
| Contents | IVD4121 |
| Purification Times | 50 Preps |
| HiPure DNA Mini Column II | 50 |
| HiPure RNA Mini Columns | 50 |
| 2ml Collection Tubes | 150 |
| Proteinase K | 24 mg |
| Protease Dissolve Buffer | 1.8 ml |
| DNase I | 600 μl |
| DNase Buffer | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Buffer RW2* | 20 ml |
| Nuclease Free Water | 10 ml |
△ Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15-25°C) does not affect their performance.
The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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