Ivd4121hipure Total Rna Kit (IVD) with Silica Gel Purification

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• Formula: /
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• Classification: Biochemical Reagents
• Grade: Bg Grade
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, For Microbiological, For Microscopic Purpose, Isolation Total Rna (Mirna)From Tissue Cell
• Model NO.: IVD4121
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Rt-PCR, Cdna Synthesis, Second Generation Sequenci
• Source: Lab Produced
• Habit Appellation: Hipure Total Rna Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Rt-PCR, Quantitative Rt-PCR, Virus DNA Test
• Main Functions: Isolation Total Rna (Mirna) From Tissue, Cell
• Applications: Rt-PCR, Cdna Synthesis, Second Generation Sequenci
• Products: Rna, Mirna
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Clinical Tissues, Cells, Lymphocytes
• Sample Amount: Tissue: <20 Mg, Cells: <5 X 106
• Yield: 2-50μg
• Elution Volume: ≥30μl
• Time Per Run: ≤25 Minutes
• After-Sale Service: Technical Support
• Sample: Free Sampe for Testing
• Used: Rt-PCR, Quantitative Rt-PCR, Test of Virus DNA
• Rapid Extraction of Rna: (Include Mirna) From Bloods, Other Clinical Sample
• Transport Package: Box
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

Product Description

HiPure Total RNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
IVD4121	HiPure Total RNA Kit	50 preps	$348.00

△ Introduction

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.




 

△ Details

Features	Specifications
Main Functions	Isolation total RNA(miRNA)from tissue, cell using two columns and DNase plus reagent
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Products	RNA, miRNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Clinical tissues, cells, lymphocytes
Sample amount	Tissue: <20 mg Cells: <5 x 10^6
Yield	2-50μg
Elution volume	≥30μl
Time per run	≤25 minutes

△ Principle

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.






△ Advantages

• Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
• High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast - the whole extraction only takes 15-25 minutes
• Nontoxic - no toxic phenol chloroform extraction is required in the extraction

△ Kit Contents

Contents	IVD4121
Purification Times	50 Preps
HiPure DNA Mini Column II	50
HiPure RNA Mini Columns	50
2ml Collection Tubes	150
Proteinase K	24 mg
Protease Dissolve Buffer	1.8 ml
DNase I	600 μl
DNase Buffer	6 ml
Buffer RTL	40 ml
RNA Digestion Buffer	15 ml
Buffer RWC*	20 ml
Buffer RW2*	20 ml
Nuclease Free Water	10 ml

△ Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15-25°C) does not affect their performance.
The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

 

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