Hipure Ffpe Rna Plus Kit Ffpe Tissue and Section Samples

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• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, for Tumor Detection Cancer Screening
• Model NO.: R414402 / R414403
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation Total Rna From Ffpe Tissue
• Source: Lab Production
• Habit Appellation: Hipure Ffpe Rna Plus Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Tumor Detection
• Property: Biochemical Reagent
• Main Functions: Isolation Total Rnafrom Ffpe Tissue/Section Sample
• Applications1: Rt-PCR, Quantitative Rt-PCR, Northern Hybridizatio
• Applications2: Poly a Purification, Nucleic Acid Protection
• Applications3: in Vitro Translation
• Products: Tumor Testing, Cancer Screening
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology, Dnase
• Process Method: Manual (Centrifugation or Vacuum)
• Adaptive Instrument: Nucleic Acid Extractor, Pipetting Workstation
• Sample Type: Ffpe Tissue Sample, Section Samples
• Sample Amount1: 6mg
• Yield: 20μg
• Elution Volume: ≥20μl
• Liquid Carrying Volume Per Column: 800µl
• Binding Yield of Column: 100µg
• Transport Package: Bottle/ Box
• Specification: 50 preps/ 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

 

Product Description

HiPure FFPE RNA Plus Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
R414402	HiPure FFPE RNA Plus Kit	50 preps	$189.00
R414403	HiPure FFPE RNA Plus Kit	250 preps	$845.00

△ Introduction

HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.







△ Details

 

Features	Specifications
Main Functions	Isolation total RNA from FFPE tissue and section samples (with DNase)
Applications	RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology, DNase
Process method	Manual (centrifugation or vacuum)
Sample type	FFPE tissue sample
Sample amount	6mg
Yield	20μg
Elution volume	≥10μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

△  Advantages

• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 60 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG
• Efficient DNA removal - unique method to effectively remove genomic DNA

△ Kit Contents

Contents	R414402	D414403
Purification Times	50 Preps	250 Preps
HiPure RNA Micro Columns	50	250
2ml Collection Tubes	50	250
Buffer DPS	60 ml	250 ml
Buffer FRL	15 ml	60 ml
Buffer RLC	15 ml	60 ml
Buffer RWC*	10 ml	50 ml
Buffer RW2*	20 ml	2 x 50 ml
DNase I	600 µl	5 x 600 µl
DNase Booster Buffer	1.5 ml	6 ml
Protease Dissolve Buffer	1.8 ml	10 ml
Proteinase K	24 mg	120 mg
RNase Free Water	10 ml	20 ml

△ Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.







 

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