Hipure Plasmid Ef Mini Kit Endotoxin-Free Plasmid DNA

• CAS No.: /
• Formula: /
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, For Microscopic Purpose, For Scintillation, Isolation 70µg Endotoxin-Free Plasmid DNA
• Model NO.: P115402/P115403
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Teaching Reagents, Cell Transfection, Animal Injection
• Source: Laboratory Production
• Habit Appellation: Hipure Plasmid Ef Mini Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Qpcr / Rt-PCR, Liquid or Solid-Phasechip Analysis
• Property: Biochemical Reagent
• Main Functions1: Isolation 70µg Endotoxin-Free Plasmid DNA
• Applications1: Cell Transfection, Animal Injection, etc.
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Conventional Plasmid
• Sample Amount1: High Copy Plasmid: 10ml Culture Medium
• Sample Amount2: Low Copy Number Plasmid : 10-15ml Culture Medium
• Yield: 10-70μg
• Elution Volume: ≥75μl
• Time Per Run: ≤40 Minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 70μg
• Sample: Free Sample
• After-Sales Service: Technical Support
• Transport Package: Box
• Specification: 50 Preps/250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

Product Description

HiPure Plasmid EF Mini Kit
 

CAT NO	PRODUCT NAME	SIZE	PRICE
P115402	HiPure Plasmid EF Mini Kit	50 preps	$99.00
P115403	HiPure Plasmid EF Mini Kit	250 preps	$448.00

△ Introduction 

The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen's innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix.

Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.





△ Details

Features	Specifications
Main Functions	Isolation up to 70µg endotoxin-free plasmid DNA from 10-15ml bacterial culture
Applications	Cell transfection, animal injection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid
Sample amount	High copy plasmid: 10ml culture medium Low copy number plasmid : 10-15ml culture medium
Yield	10-70μg
Elution volume	≥75μl
Time per run	≤40 minutes
Liquid carrying volume per column	800μl
Binding yield of column	70μg

△ Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - silica gel column purification is much faster than ion exchange
• High yield - up to 70μg plasmid can be binded in one column
• Low endotoxin content - the obtained plasmid can be directly used in cell transfection and animal injection

△ Kit Contents

Contents	P115402	P115403
Purification Times	50 Preps	250 Preps
RNase A	5 mg	20 mg
Buffer P1	30 ml	140 ml
Buffer P2	30 ml	140 ml
Buffer LEN3	15 ml	70 ml
Buffer LN4	50 ml	250 ml
Buffer LN5	30 ml	140 ml
Buffer PW1	30 ml	140 ml
Buffer PW2	12 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns III	50	250
2 ml Collection Tubes	50	250

△ Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.



 

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