Customization: | Available |
---|---|
Material: | PP |
Recommended Application: | Small Amounts of Nucleic Acid Isolation |
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CAT NO | PRODUCT NAME | SIZE | PRICE |
C13130 | HiPure DNA Plate | 10/Bag | $520.00 |
Features | Specifications |
Recommended application | Small amounts of nucleic acid isolation, viral nucleic acid from cell free samples |
Preservation conditions | Room temperature |
Stability | Up to 4 years |
Filter membrane | High quality glass fiber filter GF/F, 2 layers |
Membrane aperture | 0.7μm |
Maximum binding yield of plasmid | 30 μg |
Maximum yield of alcohol mediated Binding | 100 μg |
Single liquid carrying capacity of column | 900 μl |
Minimum elution volume | 80 μl |
Withstand centrifugal force | 5,000 x g |
Centrifuge | Low speed centrifuge, Swing out Rotor, can placed a height of 6.5cm square, (height of HiPure DNA Plate & 1.6ml Collection Plate: height, 6.2cm) |
△ Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
△ Ordering information
CAT.No. | Product Name | Package |
C13130 | HiPure DNA Plate (2 x GF/F) with 1.6ml Collection Plate |
10/Bag |
Item No. | Product Name |
Membrane type/number of layers |
Collection tubes |
Plasmid DNA binding capacity (Physical adsorption) |
gDNA/RNA binding capacity (Alcohol-mediated adsorption) |
Minimum Elution volume |
Liquid volume capacity |
C13010 | HiPure DNA Nano Column | 2 layers GF/F |
2mlwithout cap |
5μg | 20μg | 10μl | 700μl |
C13011 | HiPure DNA Micro Column | 3 layers GF/F |
2mlwithout cap |
10μg | 50μg | 15μl | 700μl |
C13100 | HiPure DNA Mini Column I | 2 layers GF/B |
2mlwithout cap |
15μg | 100μg | 30μl | 700μl |
C13110 | HiPure DNA Mini Column II | 4 layers GF/B |
2mlwithout cap |
35μg | 200μg | 50μl | 800μl |
C13111 | HiPure RNA Mini Column | 3 layers GF/B |
2mlwithout cap |
30μg | 200μg | 30μl | 800μl |
C13112 | HiPure Viral Mini Column | 3 layers GF/F |
2mlwithout cap |
30μg | 200μg | 30μl | 800μl |
C13113 | HiPure CFDNA Mini Column | 3 layers GF/F,1 layer GF/B |
2mlwithout cap |
30μg | 200μg | 30μl | 800μl |
C13120 | HiPure DNA Midi Column | 4 layers GF/B |
15mlCentrifuge tube |
125μg | 1mg | 500μl | 4ml |
C13121 | HiPure DNA Midi Column III | 8 layers GF/B |
15mlCentrifuge tube |
250μg | 1mg | 500μl | 4ml |
C13122 | HiPure DNA Maxi Column | 4 layers GF/B |
50mlCentrifuge tube |
500μg | 5mg | 1000μl | 20ml |
C13123 | HiPure DNA Maxi Column III | 8 layers GF/B |
50mlCentrifuge tube |
1mg | 5mg | 1000μl | 20ml |
C13124 | HiPure DNA Maxi Column C | 8 layers GF/B |
50ml high speedCentrifuge tube |
1mg | 5mg | 700μl | 12ml |
C13130 | HiPure DNA Plate | 2 layers GF/F | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
C13131 | HiPure gDNA Plate | 2 layers GF/B | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |