HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood

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Year of Establishment
2021-01-29
  • HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood
  • HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood
  • HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood
  • HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood
  • HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood
  • HiPure Blood DNA Mini Kit Isolation total DNA from 200μl whole blood
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Overview

Basic Info.

Model NO.
D311102 / D311103
EINECS
/
Classification
Biochemical Reagents
Grade
Reagent Grade
Specific Usage
For Biological Purpose, PCR, southern bolt and virus detection, etc
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation total DNA from 200ul Whole Blood
Source
for Lab Research
Habit Appellation
HiPure Blood DNA Mini Kit
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, Isolation total DNA from 200ul Whole Blood
Property
Biochemical Reagent
Main Functions
Isolation total DNA from 200ul Whole Blood
Applications1
PCR, southern bolt and virus detection, etc
Purification Method1
Mini spin column
Purification Technology
Silica Technology
Process Method
Manual (Centrifugation or Vacuum)
Sample Type1
Whole Blood (fresh or frozen), serum, plasma, milk
Sample Type2
other liquid samples and cultured cells
Sample Amount1
<200μl whole blood or other liquid samples
Sample Amount2
<5*106 lymphocytes or Culture Cells
Elution Volume
≥20μl
Time Per Run
≤30 minutes
Liquid Carrying Volume Per Column
800µl
Binding Yield of Column
100µg
Sample amount3
Non mammalian animals have nucleus in red blood c
Sample
Free Sample
Transport Package
Box
Specification
50 preps / 250 preps
Trademark
Magen Biotech
Origin
China

Product Description

Product Description

HiPure Blood DNA Mini Kit
 

CAT NO PRODUCT NAME SIZE PRICE
D311102 HiPure Blood DNA Mini Kit 50 Preps $99.00
D311103 HiPure Blood DNA Mini Kit 250 Preps $472.00




Introduction


Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;

3. Blood sample contains a large amount of impurities and inhibitory factors.

Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.

The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.


This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood (fresh or frozen), plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.




Details

Features Specifications
Main Functions Isolation total DNA from 200ul Whole Blood
Applications PCR, southern bolt and virus detection, etc
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Whole Blood (fresh or frozen), serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount

<200μl whole blood or other liquid samples, <5*106 lymphocytes or Culture Cells

Non mammalian animals that have nucleus in red blood cells (rich in DNA, such as birds and fish) : 5~20μl whole blood at a time

Elution volume ≥20μl
Time per run ≤30 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg

 

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).




Advantages

  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
  • Simple - all nucleic acids can be obtained by direct digestion
  • Wide applicability- handle a variety of liquid samples





Kit Contents

Contents D311102 D311103
Purification Times 50 250
HiPure DNA Mini Columns I 50 2 x 125
2ml Collection Tubes 100 5 x 100
Buffer AL 15 ml 60 ml
Buffer DW1 30 ml 150 ml
Buffer GW2* 12 ml 50 ml
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer 1.8 ml 10 ml
Buffer AE 15 ml 60 ml



Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.




Purchase Guide
 

Name CAT NO

Sample amount

Leukocyte protocol*

Colum type

Elutio volume

Average yield

Time per run

HiPure Blood DNA Mini Kit D3111 10-200μl 1ml 2ml column ≥20μl 5-9μg/200μl ≤30 minutes
HiPure Tissue&Blood DNA Midi Kit D3113 0.2-2ml 10ml 1.5ml column ≥300μl 20-40μg/1m ≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit D3115 3 -10ml 10ml 15ml column ≥700μl 20-40μg/1m ≤90 minutes
HiPure Tissue&Blood DNA 96 Kit D3117 1-200μl 1ml 96 well plate   3-8μg/200μl  


*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction






Protocol
HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood





 

Company Profile

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood

 

Exhibition all over the world

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood



 

Packaging & Shipping

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood


 

Certificate

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood

 

Laboratory

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood
 

Workshop

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood

 

Fininshed product area

 

HiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole bloodHiPure Blood DNA Mini Kit Isolation total DNA from 200&mu;l whole blood

 

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