Customization: | Available |
---|---|
CAS No.: | / |
Formula: | / |
Still deciding? Get samples of $ !
Request Sample
|
Suppliers with verified business licenses
Audited by an independent third-party inspection agency
HiPure Blood DNA Mini Kit
CAT NO | PRODUCT NAME | SIZE | PRICE |
D311102 | HiPure Blood DNA Mini Kit | 50 Preps | $99.00 |
D311103 | HiPure Blood DNA Mini Kit | 250 Preps | $472.00 |
Features | Specifications |
Main Functions | Isolation total DNA from 200ul Whole Blood |
Applications | PCR, southern bolt and virus detection, etc |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Whole Blood (fresh or frozen), serum, plasma, milk, saliva, and other liquid samples and cultured cells |
Sample amount |
<200μl whole blood or other liquid samples, <5*106 lymphocytes or Culture Cells Non mammalian animals that have nucleus in red blood cells (rich in DNA, such as birds and fish) : 5~20μl whole blood at a time |
Elution volume | ≥20μl |
Time per run | ≤30 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | D311102 | D311103 |
Purification Times | 50 | 250 |
HiPure DNA Mini Columns I | 50 | 2 x 125 |
2ml Collection Tubes | 100 | 5 x 100 |
Buffer AL | 15 ml | 60 ml |
Buffer DW1 | 30 ml | 150 ml |
Buffer GW2* | 12 ml | 50 ml |
Proteinase K | 24 mg | 120 mg |
Protease Dissolve Buffer | 1.8 ml | 10 ml |
Buffer AE | 15 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Purchase Guide
Name | CAT NO |
Sample amount |
Leukocyte protocol* |
Colum type |
Elutio volume |
Average yield |
Time per run |
HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
Protocol