Hipure Blood DNA Mini Kit Reliable PCR and Southern Blotting Total DNA (e. g., genomic, viral, mitochondrial)

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: D311102
• HS Code: 3822009090

Product Description

Product Description

Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.




Specifications

Features	Specifications
Main Functions	Isolation total DNA from 200ul Whole Blood
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Blood,serum,plasma,milk, saliva, and other liquid samples and cultured cells
Sample amount	10-250ul
Elution volume	20μl
Time per run	30 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).




Advantages

High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion,    hybridization, etc.
Fast - without separation of leukocytes, organic extraction or ethanol precipitation
Simple - all nucleic acids can be obtained by direct digestion
Wide applicability- handle a variety of liquid samples




Kit Contents

Contents	D311102	D311103
Purification Times	50	250
HiPure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	100	5 x 100
Buffer AL	15 ml	60 ml
Buffer DW1	30 ml	150 ml
Buffer GW2	12 ml	50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml

Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12months.
 

 

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