Product Description
Product Name MagPure Particles
Product specifications C14131,MP -100ml
Binding Mechanism
An appropriate lysis buffer is first added to the sample, followed by incubation with beads. The beads with bound DNA/RNA are easily pulled to the side of the test tube by applying a magnetic field, and unbound material is removed by aspiration. The magnetic separation also facilitates simple washing and elution of the isolated nucleic acids. The protocol and buffer systems used should be optimized relative to your specific sample type and target material. Magnetic-based methods are ideal for automation of high throughput processing, as they eliminate the need for centrifugation and other time-consuming steps.
Description
The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.
Main Composition
|
MagBind Paricles |
Cat.No. |
C14131 MB-100 |
Quantity |
100ml |
Concentration |
10mg/ml |
Form |
Amorphous and Porous |
Surface function |
Si-OH, Silica Beads |
Dispersion |
Polydisperse |
Particle Size |
1um |
Color |
Yellow |
Magnetic response |
Slow |
Settling Time (1ml) |
>30min |
Usage (0.2ml Sample) |
20-30ul |
DNA Recover Rate (only 4M GITC) |
<10% |
DNA Recover Rate (10% PEG8000/NaCl) |
>90% |
Recommended Use
|
1.DNA/RNA Clean Up and concentration
2.DNA/RNA Isolation from low nucleic acid content samples 3.Research immunoassays
|
Magen Kits
|
IVD3101, MD5412, D6323 |
Storage conditions and Validity
Room temperature, up to 2 years.
Features
1.Flexible: manual or automated high-throughput DNA/RNA isolation
2.Fast magnetic response: only 3 seconds for complete separation
3.High yields: up to 10 µg of genomic DNA from 200 µL of whole blood
4.Pure: isolated DNA is immediately ready-to-use in downstream application such as sequencing, in vitro transcription, restriction enzyme digestion, and or transfection of robust cell lines
5.Reproducible results and lot to lot consistency: CV < 5%
Detailed Photos
Packaging & Shipping
Magnetic beadsSemi-finished product area
Laboratory
Workshop
Fininshed product area