RNA Safer Reagent
| CAT NO |
PRODUCT NAME |
Quantity |
PRICE |
| P8310 |
RNA Safer Reagent |
500 ml |
$373.00 |
△ Introduction
RNA Safer Reagent is a safe tissue RNA protective agent. It can quickly penetrate into the cell, inactivate nuclease and protect RNA from degradation. Biological samples (tissues and cells) can be stored at room temperature (25°C) for one week, 4°C for one month, or -20°C / -80°C for long time by simply soaking in the reagent.
RNA Safer Reagent is suitable for preserving various fresh biological samples, such as animal tissues, plant tissues, cultured cells, etc. Samples protected by RNA Safer Reagent can be more convenient for transportation and storage.
△ Kit Contents
| Contents |
P8310 |
| RNA Safer Reagent |
500 ml |
△ Storage and Stability
RNA Safer Reagent can be stored at room temperature for 18 months.
If crystal precipitation occurs during storage, redissolve it in 55°C water bath.
△ Note
1. Only fresh samples can be used, cannot use frozen samples.
2. Before immersing the sample in RNASaferTM Reagent, it is necessary to process large pieces of sample into tissue blocks with side lengths less than <0.5 cm.
3. The volume of RNASaferTM Reagent shall be more than 10 times of the sample.
4. After soaking the sample in RNASaferTM Reagent, instead of stored at low temperature (- 20ºC or - 80ºC) immediately, it needs to be stored at 4ºC overnight to allow RNASaferTM Reagent to fully penetrate the tissue first.
△ Usage
1. Preservation of animal tissue
Animal tissue needs to be cut into tissue blocks with asidelength<0.5 cm, and immediately soaked in RNASafer Reagent,noneed to break up the tissue. Some small organs, such as themousekidney and spleen, can be stored entirely in the reagent.
2. Preservation of plant tissue
Some plant tissues have natural shielding, such as theparaffinlayeron the leaves, which make it difficult to be penetrated. Therefore,it is necessary to cut plant samples into small pieces or homogenizethem as much as possible to ensure that RNASaferTM Reagent canpenetrate into the tissue. Most plant samples can be directly soaked in the reagent.
3. Cultured cells
Centrifuge the cultured cells, remove the culture medium, and then add 5-10 times the volume of RNASaferTM Reagent.
4. Blood or plasma
The isolated white blood cells can be directly preserved in the RNASaferTM Reagent
5. Yeast
Centrifuge to collected 3 x 10 8 yeast cells. Discardtheculturemedium, immediately add 0.5~1 ml RNASaferTMRegent, andvortex to resuspend the cells. Yeast cells can be storedat 25ºCfor8hours, 2~8ºC for one week. For long-term preservation, after vortexplace at room temperature for 1 hour, then centrifuge at 12000xgfor 5 minutes, discard the preservation solution, quickfrozeninliquid nitrogen, and transferred to -80ºC.
6. Bacteria
Centrifuge to collect bacteria, discard the culturemedium, Escherichia coli can be stored in RNASaferTM Reagent at 2-8ºC for 1 month.
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