Hipure Gel Pure DNA Mini Kit Recover DNA Fragments Between 60bp-20kbp

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• Classification: Research Use Only
• Grade: Classic
• Specific Usage: For Biological Purpose, For Microbiological, Research Use Only
• Model NO.: D2111
• Content: Standard
• Usage: Laboratory Reagents, Teaching Reagents
• Source: Laboratory Produced
• Habit Appellation: Chemical Reagent
• Application: Research Use/for Lab
• Property: Biochemical Reagent
• Transport Package: Box
• Specification: 100 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822009090

Product Description

Product Description

Product Name HiPure Gel Pure DNA Mini Kit
Cat. No. & Specifications D2111,100Preps/kit
Introduction
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR,sequencing,restriction digestion, orvarious labeling reactions. In addition,this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.


Main Composition

Product	D2111-01	D2111-02	D2111-03
Purification times	20 Preps	100 Preps	250 Preps
Buffer GDP	20 ml	120 ml	250 ml
Buffer DW2	6 ml	2 x 20 ml	2 x 50 ml
Elution Buffer	1.5 ml	20 ml	60 ml
HiPure DNA Mini Columns I	20	100	250
2 ml Collection Tubes	20	100	250

Storage conditions and Validity
HiPure Gel Pure DNA Kits components are guaranteed for at least one year when stored at room temperature.If any precipitates form in the buffers,warm at 37ºC to dissolve.
 
 
Applicable Instrument
Nucleic Acid Extraction Machine such as KingFisher Flex, Kingfisher Duo or Similars. Automatic Nucleic acid workstation such as Tican, Hamilton, Aurora, BGI or similars.    
Sample Requirements
Virus DNA/RNA was extracted from whole blood, serum, plasma, diseased materials, feces and body fluid. If the volume of liquid sample less than 200µL, add PBS buffer or saline to 200µL.
Preparation before Use

1. According to the label, add 1.25ml(96 preps), 2.5ml(200 preps) Protease Dissolve Buffer Blue into the bottle of PK/Carrier RNA, then stored at - 20^oC after dissolve.
2. Dilute Buffer MW1 with 34ml (96 preps), 67ml (200 preps) absolute ethanol as shown on the label and store at room temperature.
3. Prepare 150ml (96 Preps) or 250ml (200Preps) 80% Ethanol using Absolute Ethanol and Nuclease-free Water, and store at room temperature.

96-Channel Nucleic Acid Extractor Operation (KingFisher Flex)

1. Add the Reagents/sample to the well of f the deep well plate according to the table below.

Name of the Plate	Pre-loaded reagents	Addition before use
Sample plate	500µl Buffer MLB 20µl MagPure Particle MPN	200µl sample 10µl PK/Carrier RNA
Wash Plate 1	500µl Buffer MW1, Put in 96 magnetic Tip
Wash Plate 2	500µl 80% ethanol
Wash Plate 3	500µl 80% ethanol
Elution plate	50~100µl Nuclease Free Water

1.  Turn on the machine, start the corresponding program. 
2.  Place the 96-well plate into the instrument as prompted.
3.  Finish the operation after ~20 minutes. Remove the 96-well plate and magnetic jacket.
4.  Store the Elute product at -20~8^oC.

Packaging & Shipping

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