Hipure Total Rna Kit From Animal Tissues, Cells and Simple Plant Tissues

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: R401102 / R401103
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Hipure Total Rna Kit
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Teaching Reagents, Isolation Total Rna (Not Include Mirna)
• Source: Laboratory Production
• Habit Appellation: Hipure Total Rna Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Animal Tissues, Cells and Simple Plant Tissues
• Property: Biochemical Reagent
• Applications1: Isolation Total Rna (Not Include Mirna)
• Applications2: All Type of Samples Can Be Used One Column
• Sample Type: Animal Tissues, Cells and Simple Plant Tissues
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology, DNA Filtration Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Amount1: Cells: ≤1 X 107
• Sample Amount2: Animal Tissue: 1-20 Mg
• Sample Amount3: Plant Leaves: 50-150 Mg
• Yield: 2-100μg
• Elution Volume: ≥50μl
• Time Per Run: ≤15 Minutes (1-24 Samples)
• Liquid Carrying Volume Per Column: 800µl
• Binding Yield of Column: 100µg
• Sample: Free Sample
• Transport Package: Box
• Specification: 50 Preps/ 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 90279000
• Production Capacity: 100000 Per Month

Product Description

Product Description

 

HiPure Total RNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
R401102	HiPure Total RNA Kit	50 preps	$99.00
R401103	HiPure Total RNA Kit	250 preps	$448.00

△ Introduction

The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.





△ Details

Features	Specifications
Main Functions	Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications	RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount	Cells: ≤1 x 10^7 Animal tissue: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 x 10^6
Yield	2-100μg
Elution volume	≥50μl
Time per run	≤15 minutes(1-24 samples)
Liquid carrying volume per column	800µl
Binding yield of column	100µg

△ Advantages

• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 20 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG

△ Kit Contents

Contents	R401102	R401103
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
RTL Lysis Buffer	50 ml	200 ml
RNA Binding Buffer	15 ml	75 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

△ Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


 

 

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