| Customization: | Available |
|---|---|
| Material: | Plate,Liquid,Powder |
| Transport Package: | Bottle |
Suppliers with verified business licenses
Audited Supplier Audited by an independent third-party inspection agency
Introduction
RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphategroup attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2',3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. The highest activity is exhibited with single-stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.
Specifications
| Features | Specifications |
| Appearance | White or light yellow lyophilized powder |
| Molecular weight | 13.7 KDa |
| Purity | ≥60% RNase A (SDS-PAGE) |
| Specific activity | >50 Kunitz units/mg protein |
| Preservation conditions | -20-8°C. RNase is stable under heating and detergent conditions and can withstand high temperature treatment of 100°C. |
| DNase residue detection | Not detected. In 10μl (including 200ng plasmid) solution, RNase A was added to the final concentration of 50-100 μg/ml and placed at room temperature for 30 minutes. After electrophoresis, the main band was obvious without degradation. |
| Application | Remove RNA from preparations of plasmid DNA |
Advantages
| Contents | C12120 | C12121 |
| RNase A, Lyophilizate, >50 U/mg of protein | 1 g | 10 g |
){kind=link}