| Material: | PP |
|---|---|
| Plasmid Yields: | up to 0.25mg |
| Recommended Application: | Plasmid Medium Yield Preparation |
| Preservation Conditions: | Room Temperature |
| Stability: | up to 4 Years |
| Filter Membrane: | High Quality Glass Fiber Filter GF/B, 8 Layers |
| Samples: |
|---|
| Customization: |
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Suppliers with verified business licenses
Audited Supplier HiPure DNA Midi Column III
(15ml, 8 layers of GF / B imported glass fiber filter membrane)
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| C13121 | HiPure DNA Midi Column III | 100/Bag | $572.00 |
△ Introduction
Magen's HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen's columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
△ Details
| Features | Specifications |
| Recommended application | Plasmid Medium Yield preparation |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 8 layers |
| Membrane aperture | 1.0 μm |
| Maximum binding yield of plasmid | 250 μg |
| Maximum yield of alcohol mediated Binding | 1 mg |
| Plasmid Yields | Up to 0.25mg |
| Single liquid carrying capacity of column | 4 ml |
| Minimum elution volume | 500 μl |
| Withstand centrifugal force | 5,000 x g |
| Centrifuge | Lowspeed centrifuge for 15ml centrifuge tubes, >3000 x g,swing-out Rotor, or Fixed Angle Rotor |
△ Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
△ Ordering information
| CAT.No. | Product Name | Package |
| C13121 | HiPure DNA Midi Column III (8 x GF/B) with 15ml Collection Tubes |
100/Bag |
| Item No. | Product Name |
Membrane type/number of layers |
Collection tubes |
Plasmid DNA binding capacity (Physical adsorption) |
gDNA/RNA binding capacity (Alcohol-mediated adsorption) |
Minimum Elution volume |
Liquid volume capacity |
| C13010 | HiPure DNA Nano Column | 2 layers GF/F |
2mlwithout cap |
5μg | 20μg | 10μl | 700μl |
| C13011 | HiPure DNA Micro Column | 3 layers GF/F |
2mlwithout cap |
10μg | 50μg | 15μl | 700μl |
| C13100 | HiPure DNA Mini Column I | 2 layers GF/B |
2mlwithout cap |
15μg | 100μg | 30μl | 700μl |
| C13110 | HiPure DNA Mini Column II | 4 layers GF/B |
2mlwithout cap |
35μg | 200μg | 50μl | 800μl |
| C13111 | HiPure RNA Mini Column | 3 layers GF/B |
2mlwithout cap |
30μg | 200μg | 30μl | 800μl |
| C13112 | HiPure Viral Mini Column | 3 layers GF/F |
2mlwithout cap |
30μg | 200μg | 30μl | 800μl |
| C13113 | HiPure CFDNA Mini Column | 3 layers GF/F,1 layer GF/B |
2mlwithout cap |
30μg | 200μg | 30μl | 800μl |
| C13120 | HiPure DNA Midi Column | 4 layers GF/B |
15mlCentrifuge tube |
125μg | 1mg | 500μl | 4ml |
| C13121 | HiPure DNA Midi Column III | 8 layers GF/B |
15mlCentrifuge tube |
250μg | 1mg | 500μl | 4ml |
| C13122 | HiPure DNA Maxi Column | 4 layers GF/B |
50mlCentrifuge tube |
500μg | 5mg | 1000μl | 20ml |
| C13123 | HiPure DNA Maxi Column III | 8 layers GF/B |
50mlCentrifuge tube |
1mg | 5mg | 1000μl | 20ml |
| C13124 | HiPure DNA Maxi Column C | 8 layers GF/B |
50ml high speedCentrifuge tube |
1mg | 5mg | 700μl | 12ml |
| C13130 | HiPure DNA Plate | 2 layers GF/F | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
| C13131 | HiPure gDNA Plate | 2 layers GF/B | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
Suppliers with verified business licenses
Audited Supplier 
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