Magzol Reagent Isolation of Total Rna From Cells and Tissues

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: R480101 / R480102
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Magzol Reagent
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Teaching Reagents, Rna Isolation Solvent (Substitution Trizol/Qiazol
• Source: Laboratory Production
• Habit Appellation: Magzol Reagent
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Animal Tissues, Cells and Simple Plant Tissues
• Property: Biochemical Reagent
• Applications1: Rna Isolation Solvent (Substitution Trizol/Qiazol
• Applications2: Rt-PCR, Northern Hybridization, Poly (a) Enrichmen
• Purification Technology: Acid Phenol Guanidine Isothiocyanate
• Process Method: Manual (Centrifugation)
• Sample Type1: Animal Tissue, Plant Tissue, Adherent Cells
• Sample Type2: Suspended Cells, Bacteria, Trace Fungi
• Sample Amount1: Flexible
• Purification Method: Mini Spin Column 96 Well Plate
• Elution Volume: Variation with Sample Size
• Time Per Run: Variation with Sample Size
• Sample: Free Sample
• After-Sales Service: Technical Support
• Transport Package: Box
• Specification: 100ml / 200ml
• Trademark: Magen Biotech
• Origin: China
• HS Code: 90279000
• Production Capacity: 100000 Per Month

Product Description

Product Description

 

MagZol Reagent

CAT NO	PRODUCT NAME	SIZE	PRICE
R480101	MagZol Reagent	100 ML	$79.00
R480102	MagZol Reagent	200 ML	$149.00

△ Introduction

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi.

The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.


This method is suitable for small quantities of tissue (<100mg) and cells (<5 X10⁶), and large quantities of tissue (up to 1g) and cells (<10⁸), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour.

Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.





△ Details
 

Features	Specifications
Main Functions	RNA isolation solvent (substitution for Trizol/Qiazol reagent)
Applications	RT-PCR, Northern hybridization, poly (a) enrichment, etc.
Purification technology	Acid phenol guanidine isothiocyanate
Process method	Manual (centrifugation)
Sample type	Animal tissue, plant tissue, adherent cells, suspended cells, bacteria, trace fungi, etc.
Sample amount	Flexible
Elution volume	Variation with sample size
Time per run	Variation with sample size

△ Advantages

• Extensive - suitable for various kinds of biological samples, including animals, plants, cultured cells, bacteria, etc.
• High yield - efficient cleavage releases more RNA
• High purity - purified RNA is suitable for various downstream applications
• Flexible - sample amount can be adjusted according to the demand
• High cost performance compared with similar products

 

△ Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.




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