Product Description
HiPure Paxigene Blood RNA Kit
CAT NO |
PRODUCT NAME |
SIZE |
PRICE |
R416802 |
HiPure Paxigene Blood RNA Kit |
10 preps |
$99.00 |
R416803 |
HiPure Paxigene Blood RNA Kit |
50 preps |
$373.00 |
△ Introduction
The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
△ Details
Features |
Specifications |
Main Functions |
Isolation total RNA from Paxgene RNA Tubes |
Applications |
RT-PCR, qPCR, Northern hybridization, NGS, nucleic acid protection, in vitro translation |
Purification method |
Mini spin column |
Purification technology |
Silica technology, DNA filtration technology |
Process method |
Manual (centrifugation or vacuum) |
Sample type |
Blood in the preservation tube |
Sample amount |
2.5ml |
Elution volume |
≥30μl |
Liquid carrying volume per column |
800µl |
Binding yield of column |
100μg |
△ Advantages
- High quality - high purity total RNA can be directly used in various sensitive downstream applications
- Fast - isolation of several samples can be completed in 40 minutes by using column purification method
- Safety - no phenol chloroform extraction required
- Sensitive - direct lysis of blood, plasma and other samples without separation of leukocytes
△
Kit Contents
Contents |
R416802 |
R416803 |
Purification Times |
10 Preps |
50 Preps |
HiPure RNA Mini Columns I |
10 |
50 |
gDNA Filter Mini Columns |
10 |
50 |
2ml Collection Tubes |
30 |
150 |
RNase Free Water |
60 ml |
250 ml |
Buffer MBR1 |
10 ml |
30 ml |
Buffer MBR2 |
5 ml |
15 ml |
Buffer RW1 |
15 ml |
60 ml |
Buffer RW2* |
6 ml |
20 ml |
Proteinase K |
12 mg |
50 mg |
Protease Dissolve Buffer |
1.8 ml |
5 ml |
DNase I |
120 µl |
600 µl |
DNase Buffer |
6 ml |
30 ml |
△
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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