Hipure Plasmid Ef Maxi Kit

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• Classification: for Lab
• Grade: Classic
• Specific Usage: For Biological Purpose, for Lab Research
• Model NO.: P115602
• Content: Standard
• Usage: Laboratory Reagents
• Source: for Lab Research
• Habit Appellation: Chemical Reagent
• Application: for Lab Research
• Property: Neutral
• Transport Package: Box
• Specification: 10 Preps/Kit
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

Product Name HiPure Plasmid EF Maxi Kit
Cat. No. & Specifications  P115602,10 Preps/Kit  
Introduction
The HiPure Plasmid EF Maxi Kit combine the power of HiPure technology with Magen's innovative Endotoxin Removal Technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.





Main Composition

Product Number	P115602	P115603
Purification Times	10 Preps	50 Preps
RNase A	30 mg	150 mg
Buffer P1	100 ml	500 ml
Buffer P2	100 ml	500 ml
Buffer LN3	50 ml	250 ml
Buffer PW1	33 ml	180 ml
Buffer PW2	20 ml	100 ml
Elution Buffer	15 ml	120 ml
Buffer CL	33 ml	180 ml
HiPure DNA Maxi Columns C	10	50
Lysate Clear Midi Syringe	10	50
50 ml Collection Tubes C	20	100

Storage conditions and Validity
HiPure Plasmid DNA Kits components are guaranteed for at least one year when stored at room temperature.
If any precipitates form in the buffers,warm at 37ºC to dissolve.
After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C.




Materials and Equipment to be Supplied by User

1. Dilute Buffer PW2 with 100% ethanol and store at room temperature

2. Add the vial of RNase A to the bottle of Buffer P1 and store at 2-8˚C

3. Heat Elution Buffer to 70°C if plasmid DNA is >10kb

 

 

Packaging & Shipping

 

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