Magpure Viral Nucleic Acid Isolation Kit Ivd5412

• Transport Package: Box
• Specification: 200 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: IVD5412
• HS Code: 3822009090

Product Description

Magen's Nucleic Acid Isolation &Purification Kits Germany in-Vitro Diagnostic registered (98/79/EC) Chinese in vitro diagnostic Reagent Glass I, 20150062)
Function Name	Cat.No.	Quality (Preps)	Price	Nucleic Acid Type	Method	Sample	Applicaton
HiPure Universal DNA Kit	IVD3018	100		genomic DNA	Silica-gel Column	10~200ul Blood, Plasma, Saliva, Body fluids 1~20mg Tissue, <3 x 10^6 Culture Cells Dry Blood Spot and swab FFPE sample	PCR, Real time PCR NGS Library prepare
HiPure Circulating DNA Kit	IVD3182	50		Circulating DNA		1~5 ml Plasma, Serum, cerebral effusion, ascites, cell culture supernatants.	PCR, Real time PCR NGS Library prepare
HiPure Viral DNA/RNA Kit	IVD4173	100		Viral Nucleic Acid		~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant, other body fluids	Real Time PCR PCR
HiPure Viral RNA Kit	IVD4175	100		Viral RNA		~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant , other body fluids	Real time PCR PCR
MagPure Universal DNA Kit	IVD3102	200		genomic DNA	Magnetic Particles	10~200ul Blood, Plasma, Saliva, Body fluids 1~20mg Tissue, <3 x 10^6 Culture Cells Dry Blood Spot and swab FFPE sample	PCR, Real time PCR NGS Library prepare
MagPure Viral DNA/RNA Kit	IVD5412 (Bottle package)	200		Viral Nucleic Acid		~200ul Plasma, Serum, Swab Soak, Tissue homogenate superatant, other body fluids	Real Time PCR PCR
	IVD5412-F-96 (Precast for kingFisher Flex)	96
	IVD5412-TL-96 (Precast for 32 channel Machine)	96
MagPure Stool DNA Kit	D6364-400	400		Stool DNA		Isolation Total DNA include microbial DNA from ~150mg Stool Samples.	PCR, Real time PCR NGS Library prepare

 

Product Description

Product Name MagPure Viral Nucleic Acid Isolation Kit
Cat. No. & SpecificationsIVD5412, 96 Preps/Kit; 200 Preps/Kit

Intended Use
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.


Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.


Main Composition

	96 preps	200 preps	Contents
MagPure Particles N	2.5 ml	5 ml	Magnetic Bead/NaCl/NaN3
PK/Carrier RNA	25 mg	50 mg	Protease
Protease Dissolve Buffer Blue	5 ml	5 ml	Glycerol/CaCl2/Tris
Buffer MLB	60 ml	120 ml	Guanidine Salt/Isopropanol/EDTA
Buffer MW1	26 ml	53 ml	Guanidine Salt/Tris/EDTA
Nuclease Free Water	15 ml	30 ml	10mm Tris, pH8.0

Storage conditions and Validity
This kit is shipped and stored at room temperature and is valid for 12 months.

Applicable Instrument
Nucleic Acid Extraction Machine such as KingFisher Flex, Kingfisher Duo or Similars. Automatic Nucleic acid workstation such as Tican, Hamilton, Aurora, BGI or similars.    


Sample Requirements
Virus DNA/RNA was extracted from whole blood, serum, plasma, diseased materials, feces and body fluid. If the volume of liquid sample less than 200µL, add PBS buffer or saline to 200µL.


Preparation before Use

1.According to the label, add 1.25ml(96 preps), 2.5ml(200 preps) Protease Dissolve Buffer Blue into the bottle of PK/Carrier RNA, then stored at - 20^oC after dissolve.

2.Dilute Buffer MW1 with 34ml (96 preps), 67ml (200 preps) absolute ethanol as shown on the label and store at room temperature.

3.Prepare 150ml (96 Preps) or 250ml (200Preps) 80% Ethanol using Absolute Ethanol and Nuclease-free Water, and store at room temperature.

96-Channel Nucleic Acid Extractor Operation (KingFisher Flex)

1.Add the Reagents/sample to the well of f the deep well plate according to the table below.

Name of the Plate	Pre-loaded reagents	Addition before use
Sample plate	500µl Buffer MLB 20µl MagPure Particle MPN	200µl sample 10µl PK/Carrier RNA
Wash Plate 1	500µl Buffer MW1, Put in 96 magnetic Tip
Wash Plate 2	500µl 80% ethanol
Wash Plate 3	500µl 80% ethanol
Elution plate	50~100µl Nuclease Free Water

2. Turn on the machine, start the corresponding program. 

3.Place the 96-well plate into the instrument as prompted.
4.Finish the operation after ~20 minutes. Remove the 96-well plate and magnetic jacket.

5. Store the Elute product at -20~8^oC.

Packaging & Shipping

Certificate

Laboratory

Workshop

Fininshed product area