| Customization: | Available |
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| CAS No.: | / |
| Formula: | / |
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HiPure Plasmid EF Midi Kit
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| P123102 | HiPure Plasmid EF Midi Kit(Vacuum) | 50 preps | $423.00 |
| P123102B | HiPure Plasmid EF Midi Kit(Spin) | 50 preps | $547.00 |
△ Details
| Features | Specifications |
| Main Functions | Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture |
| Applications | Cell transfection, animal injection, etc. |
| Purification method | Midi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Conventional plasmid, plasmid less than 30KB |
| Sample amount | 25-50ml |
| Yield | 10-500μg |
| Elution volume | ≥0.4ml |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 250μg |
△ Advantages
△ Kit Contents
| Contents | P123102 | P123102B |
| Purification Times | 50 Preps | 50 Preps |
| RNase A | 30 mg | 30 mg |
| Buffer E1 | 150 ml | 150 ml |
| Buffer E2 | 150 ml | 150 ml |
| Buffer E3 | 150 ml | 150 ml |
| Buffer E4 | 150 ml | 150 ml |
| Buffer E5 | 150 ml | 150 ml |
| Buffer PW2* | 50 ml | 50 ml |
| Elution Buffer | 30 ml | 30 ml |
| MaxPure EF Mini Column | 50 | 50 |
| 2 ml Collection Tubes | 50 | 50 |
| Lysate Clear Midi Syringe | 50 | 50 |
| Extend Tubes | 50 | 50 |
| 50ml Centrifuge Tubes | 50 | |
| Support Tubes | 50 |
△ Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C.
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