Hipure Bacterial DNA Kit From Cultures / Food

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D314602 /D314603
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, PCR, Southern Blot and Enzyme Digestion
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation Bacterial DNA From Cultures, Food
• Source: for Lab Research
• Habit Appellation: Hipure Bacterial DNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Enzyme Digestion, Sequencing, PCR, Cloning
• Property: Biochemical Reagent
• Main Functions: Isolation Bacterial DNA From Cultures, Food
• Applications1: PCR, Southern Blot and Enzyme Digestion, etc.
• Purification Method1: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Culture Medium, Swab, Parasitic Blood, Tissue,Sput
• Sample Amount1: Bacterial Culture Medium: 0.5-2 Ml
• Sample Amount2: Tissue Samples: 50-100 Mg
• Sample Amount4: Viscous Secretion: 0.1-1 G
• Sample Amount3: Whole Blood / Cell Suspension: 0.5-1 Ml
• Elution Volume: ≥30μl
• Time Per Run: ≤40 Minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Sample: Free Sample
• Transport Package: Box
• Specification: 50 preps/250 preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

HiPure Bacterial DNA Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
D314602	HiPure Bacterial DNA Kit	50 preps	$149.00
D314603	HiPure Bacterial DNA Kit	250 preps	$646.00

△ Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.

△ Details

Features	Specifications
Main Functions	Isolation bacterial DNA from cultures, food and other samples
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture medium, swab, parasitic blood, tissue,sputum, etc.
Sample amount	Bacterial culture medium: 0.5-2 ml Tissue samples: 50-100 mg Whole blood / cell suspension: 0.5-1 ml Viscous secretion: 0.1-1 g
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

△  Advantages

• Fast - several samples can be extracted in 40 minutes (after digestion)
• High purity - purified DNA can be directly used in various downstream applications
• Good repeatability - silica technology can obtain ideal results every time
• High recovery - DNA can be recovered at the level of PG
• Broad spectrum - it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
• Sufficient components - this kit has carried lysozyme, protease K, RNase A and glass beads

△ Kit Contents

Contents	D314602	D314603
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	50	2 x 125
Glass Beads (0.1~0.2mm)	20 g	100 g
Buffer P1	20 ml	100 ml
Buffer DL	15 ml	80 ml
Buffer GW1	22 ml	88 ml
Buffer GW2	12 ml	50 ml
Lysozyme	60 mg	300 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml

△ Storage and Stability

Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.




 

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