Hipure Fruit Rna Kit

• Customization: Available
• Type: External Use
• Transport Package: Box
• Model NO.: R401402
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• HS Code: 90279000
• Production Capacity: 100000 Per Month

Product Description

Product Description

Introduction
The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA.


There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purification, nuclease protection and in vitro translation.




Specifications

Features	Specifications
Main Functions	Isolation total RNA from 150 mg fruit or seed using Plantzol reagent
Applications	RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation
Purification method	Mini spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	Simple plant, petal, peel, seed
Sample amount	50-150mg
Elution volume	≥300μl
Time per run	≤15 minutes(1-24 samples)
Liquid carrying volume per column	800µl
Binding yield of column	100µg

 

Principle

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized.


The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.




Advantages

• Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
• High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast - several samples can be extracted in 15 minutes by column method
• Wide range - samples including animals, plants, bacteria, cells, etc
• High output - large sample amount, and the yield can be up to 200μg

Kit Contents

Contents	R401402	D401403
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
PlantZol Reagent	60 ml	200 ml
Buffer GXP	30 ml	150 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

 

Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.




 

Certifications

 

Packaging & Shipping

 

Fininshed product area

Laboratory

Workshop