Hipure Plasmid Mini Kit II

• CAS No.: /
• Formula: /
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, Enzyme Digestion, Sequencing, PCR, Cloning
• Model NO.: P100202/P100203
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Isolation 70μg Plasmid DNA 5-15ml Bacterial
• Source: for Lab Research
• Habit Appellation: Hipure Plasmid Mini Kit II
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Enzyme Digestion, Sequencing, PCR, Cloning
• Property: Biochemical Reagent
• Main Functions: Isolation 70μg Plasmid DNA 5-15ml Bacterial cUL
• Applications1: Enzyme Digestion, Sequencing, PCR, Cloning, etc.
• Purification Method1: MIDI Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type1: Conventional Plasmid, Plasmid Less Than 30kb
• Sample Amount1: High Copy Plasmid: 1-5ml Culture Medium
• Yield: 20-80µg
• Elution Volume: ≥75μl
• Time Per Run: Complete 1-24 Samples in 30 Minutes
• Liquid Carrying Volume Per Column: 800µl
• Binding Yield of Column: 70µg
• After-Sales Service: Technical Support
• Sample: Free Sample
• Shipping: FedEx, DHL, Door to Door
• Transport Package: Box
• Specification: 50 preps/250 preps
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

HiPure Plasmid Mini Kit II

CAT NO	PRODUCT NAME	SIZE	PRICE
P100202	HiPure Plasmid Mini Kit II	50 preps	$60.00
P100203	HiPure Plasmid Mini Kit II	250 preps	$273.00

△ Introduction

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.

Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

△ Details

Features	Specifications
Main Functions	Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR, cloning, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	High copy plasmid: 1-5ml culture medium Low copy plasmid : 5-10ml culture medium
Yield	20-80µg
Elution volume	≥75μl
Time per run	Complete 1-24 samples in 30 minutes
Liquid carrying volume per column	800µl
Binding yield of column	70µg

△  Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
• High yield - up to 70µg plasmid can be binded in one column

△ Kit Contents

Contents	P100202	P100203
Purification Times	50 Preps	250 Preps
RNase A	5 mg	20 mg
Buffer P1	30 ml	140 ml
Buffer P2	30 ml	140 ml
Buffer P3	40 ml	200 ml
Buffer PW1	30 ml	140 ml
Buffer PW2*	12 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns III	50	250 ml
2 ml Collection Tubes	50	250 ml

△ Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C.


 

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